East Bay

Would it make sense to try SoulCycle within the Berkeley Equinox? The original location was over by Gilman, and it seems like parking over there is more of a pain than Shattuck.

For the WCRK riders, it’s not as ideal because of 24/ the Caldicott tunnel, but is it better than nothing?

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u/hsgual — 3 days ago

What color shoes to match this?

The suit is currently at the tailor to lengthen the pants, but I tried to get a close up of the material. It has a subtle check pattern that is hard to see. I have dark chocolate brown dress shoes, but the vibe is off (also the wrong socks in the photo).

Oxblood for these? Or another shade of brown?

u/hsgual — 5 days ago

Return Policy Clarification

The Real Real has a three strike policy with returns stating the following:

“The RealReal operates on a three strike policy for clients who exhibit high-risk return behavior. High-risk behavior is identified as:
- Returning 30 or more items
- Returning purchases greater than $10K
- Overall return rate of 85%

Strikes are not assessed in instances of TRR fault (example, an item was damaged or incorrectly listed).

If a strike is assessed, the client will be notified via email. The strike will automatically fall off the account after 12 months.

Clients who reach three strikes will be prohibited from making any returns, regardless of the reason, until the strikes fall off the account.”

If I generally return an item it’s because the listings measurements and the garment size don’t agree, or it was listed incorrectly. So is that counting against me with their return policy?

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u/hsgual — 8 days ago

Lengthen the pants?

I went second hand shopping and found this wonderful Zegna suit. Everything fits well, but my gut reaction is the pants need to be lengthened. Thankfully looking, it seems that the hemming can be undone (and seems to be hand stitched).

These aren’t proper socks for the shoes, but were to get a quick photo. And yes, these shoes need a polish and are not entirely right for this suit.

u/hsgual — 13 days ago

How reliable are measurements?

I ask because I had two suits sent from TRR. One fits incredibly well, one is too small. So now I am trying to see if I can line up the measurements as to why, and find others to try on from the site.

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u/hsgual — 14 days ago
▲ 7 r/Cello

Cassado Help

I was wondering if anyone had fingerings for these chords in the Toccata by Cassado? This is the one section I am having issues mapping…

u/hsgual — 14 days ago
▲ 60 r/Canning

No canning for me this year.

Last year I went to the farmers market, picked through all of the cosmetic seconds and then proceeded to make apricot jam from close to 60 lbs of Blenheim Apricots. I also then made a version of the NCHFP peach and orange marmalade that swapped peaches for pineapple, and used limes and lime juice for acidity instead of lemon. I also made grapefruit marmalade with ginger. All in all, I canned so much last year I didn’t have the mental energy to do it this year.

I give props to those who do this year in and year out as a ritual.

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u/hsgual — 18 days ago
▲ 6 r/wine

A just meh Sauternes

I had four bottles of this gifted to me. We finished this 500 mL bottle, but whatever we have left will likely be used in baking projects 🙈.

This Sauternes isn’t… great. It’s not balanced. Orange marmalade, complete with pith, a little bit of custard, and a blend of stone fruits like apricot and peach. But the acidity to me simply isn’t there, and it’s hard to parse presence of oak for added depth. It’s incredibly sweet, and comes across sweeter, but not denser, than L'extravagant De Doisy Daene.

I’m aware this Sauternes is inexpensive after searching. However, there are equivalently priced or slightly more priced Grand Crus that punch above their weight and are preferred.

u/hsgual — 24 days ago
▲ 3 r/Cello

Saint Saens tips (3rd movement)

Context: I’m an adult student who has studied for a long time (like 20 years since 10) but I did not go to conservatory.

Im in between lessons during the summer (I’ll see my teacher in 1.5 weeks), and I’ve been working on the Saint Saens concerto for the last four months. Where I am running into some difficulty is the sixths (after rehearsal mark L).

My first approach has been to first look at which chord is being explored, and make sure the intervals are correct. But where I am running into trouble is getting the shift to be accurate between groups of notes.

I’m trying

  1. Each “voice” separately, usually in the context of the chord playing as a drone.
  2. Doing the above with dotted rhythms and impulse groups.
  3. Slowly trying to put it together, but I’m finding in some spots the pitch could be better centered.

I am also somewhat playing with right arm pronation to avoid flapping my wings as much as little elbow circles.

So I am wondering what I need to adjust…. Does anyone have suggestions for nailing this movement?

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u/hsgual — 28 days ago

How to make kitchen feel less heavy.

I feel like to make our kitchen feel less “heavy,” the counter and backsplash needs to change. I also don’t feel like they go with the cabinets. BUT, my partner doesnt want to part with the countertops as they are granite… and instead wants to consider having the cabinets and shelved painted. From digging around this sub, it seems to be not advised.

Where I need help is, if the backsplash and counters were to change what color to consider given how strong the cabinet stain is?

u/hsgual — 1 month ago
▲ 40 r/wine

1984 Chateau de Fargues

Wow… I was surprised to see that this Sauternes had so much acidity and vibrancy, with peach, apricot, and pineapple leading on the palette. Not overly sweet at all, and only a hint of orange marmalade, ginger, and raisin. It honestly has tasted similar to younger Sauternes I have had lately, albeit with a stronger mouth feel and much longer finish. A flier from an auction lot that paid off.

u/hsgual — 2 months ago

ddPCR optimization help

Throwing this out to my fellow labrats as a follow up because I’m even stumped:

We are working on a ddPCR titer assay, and for the life of us we cannot figure out why we are seeing over- recovery with sets of primer/probes. Early phase assays usually accept 70-130% recovery. First pilot, recovery was 150%, and that came down by simply re measuring the gBlock control with nanodrop. Now we have some runs clocking in at 125% across all six dilution levels, others where across 2/6 it’s 100-110%, and then 4/6 spike to 135%.

Things we have done: screened the handful of assays we could design given sequence constraints, optimized for annealing/ extension to minimize rain, showed that the reactions hit endpoint by cycle 40 vs 45. We have also showed that the candidate assay is specific to our target, no cross reactivity against other constructs or human genomic DNA.

- is it possible the nanodrop is still not precise enough to close the gap? Or is there something in our reaction conditions we could optimize further?

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u/hsgual — 2 months ago

ddPCR and qPCR in GMP

For my fellow r/labrats that work in GMP analytical spaces… what is the smallest volume you would pipette for a ddPCR/dPCR or qPCR workflow? Including in sample prep/ serial dilutions?

I am trying to standardize workflows across our team, and I suspect that low volume pipetting is causing some accuracy problems and apparent over-recovery in one of our digital PCR assays. We aren’t GMP, but wondering if there are best practices we could incorporate early.

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u/hsgual — 2 months ago

AAV Capsid ELISA question

I am testing two different AAV capsid ELISAs and getting different results between them using the same externally produced material.

Kit A: calls for a sample Lysis, adding I think an SDS based regent and heating the capsids to 99C. It seems odd, and I’ve never seen other capsid titer ELISAs do this. The vendor also doesn’t provide much CoA around the standard employed.

Kit B: assumes capsids are intact, and this seems to be more standard format when I survey vendors. It’s also from a more typical AAV vendor.

Kit B agrees with qPCR and Mass photo data (for empty full). Kit A does not, and is 4-5x higher.

We need to decide which method to proceed with, and I’m wondering what others have seen as being a more typical format?

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u/hsgual — 2 months ago
▲ 3 r/wine

Champagne recommendations

For my birthday a few years ago, I was given a few bottles of Piper Hiedsieck, 2006 Brut Vintage. I’ve been slowly opening them over the last four years, and I really enjoy the flavors here. Getting hints of coffee, bitter almond and brioche along side the green apple, pear, and grapefruit.

Does anyone have suggestions on where to go next, and is this a profile likely to only be found with vintage releases?

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u/hsgual — 2 months ago
▲ 37 r/biotech

Additional Genentech restructuring?

Random question: is Genentech forcing legacy employees to retire? I am seeing so many LinkedIn posts with a theme of “it’s bittersweet I’m announcing my retirement.”

I heard rumors gRED is under much more scrutiny for having ran too many projects that spent R&D $$$ that yielded no new molecules into the pipeline….

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u/hsgual — 2 months ago

River Oaks to IAH

Im hoping to ride in River Oaks during a business trip to Texas. If I did the 6 AM class… how long would it take to get to IAH at about 7:30 in the morning? How is the locker room situation?

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u/hsgual — 3 months ago
▲ 18 r/AskSF

Selling clothes to Crossroads

Has anyone here sold clothes with Crossroads know how stringent their designer policy? For example; I have unworn shirts from Calvin Klein with the tags still on. But would they deny them because Calvin Klein is not on their targeted list? (Yet J Crew is).

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u/hsgual — 3 months ago

Feeling lost in a LTR

I feel like I go through these phases a two to three times a year where I am very uncertain if my relationship is going to make it.

Myself and my partner, who is 15 years older than I, have been together for 10 years. It’s kind of been a rollercoaster, in that I would say 70% of the time has been positive where our relationship is stable. But the remaining 30% has been heavy — navigating infidelity on his end, and navigating how he stole, and then settled out of court legally, from a previous employer.

After the cheating and such early on in our relationship, we sought couples therapy and figured out a lot of the core issues. There was a good stretch where I felt things were balanced, we were planning for the future, and I was legitimately happy. Communication was working, our sex life felt reasonable, and it felt like we could tackle anything. We even bought a home together. I feel like after the second break of trust a few years back it’s been more of the up and down.

I’m not perfect, in that I can sometimes become checked out. This is especially after a crazy work week at a lean start up. The rough patches for me are also when I become withdrawn at home. I sometimes am tired of feeling as if I am the one who has to manage the emotional world of our entire relationship. As an example, couples therapy gave us tools for relationship check-ins… said check-ins only happen if I initiate them.

He’s come to me more lately saying “I need to lead more activities and plans at home.” It’s valid feedback to work from, and I’ll do my best to make that happen. Some of it feels like mind reading, but it’s becoming clear that when certain suggestions come up it’s not optional it’s his way of saying “please execute.”

I feel like intimacy issues have come back again, and sex has become infrequent and only tailored towards his pleasure. When he wants intimate moments that are non sexual, I can feel myself get tense instead of relax. There are thoughts of like “what now?” Or “when is the other shoe going to drop.” It’s surprising because I didn’t feel this way five years ago.

I feel like I’m at a crossroads, where I can see my life moving forward in this relationship and changing it OR this thing fizzles out in the next five years and we go separate ways. I think that’s the hard part because I clearly have a decision to make. I have therapy next week, which will help.

But basically, I’m curious if those who have been partnered long term have navigated similar decisions and what did you do?

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u/hsgual — 3 months ago
▲ 1 r/wine

Wine fridge recommendations

We are looking for a second wine fridge that will be used for long term storage. We like to assemble verticals ourselves from small local wineries, and as well from travels. On average we tend to have over 100 bottles on hand, split between drink now (60%) and drink later (40%). I prefer freestanding, and an opaque door (like in my lab where we store UV sensitive materials). It would be placed in a cooler area, but this home doesn’t have central air.

Aside from the Eurocave family, are there other recommendations? Located in SF if that helps.

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u/hsgual — 3 months ago