There is a really good Vietnamese restaurant that imports this brewery that I really like. I'm loving the beer but don't particularly want to pay $9 plus tip for a single beer.
Anyone know if other places distribute this brewery?
I have an old TSQ Altis with a thermo ultimate 3000 for the hplc.
While running my method, I started to see some tailing and poor peak shapes. There was a leak where the guard column and line from the injector. I assumed that was the problem and replaced the guard column. Now after the first injection, the RT shifts from 1.54 to 1.13 as well as tailing and about 20-30% loss in peak sensitivity. I've never seen a run that had it's first injection great then all subsequent injections poor.
I've tried a new column, a different used column, no guard column, running on a different channel, changing peek tubing, back flushing columns with IPA, purging both channels and all lines even the unused ones. Even injecting a 96 well plate with no cover in case the needle was getting clogged. Flushed the whole system and finally coming to my wits end. I thought if there was air in the line somewhere I would have dislodged it by now.
Other symptoms are that the first injection starts at 3500 psi instead of the method average of 2700 psi. The first injection pressure line follows the same pattern as the method average just at a higher psi. The second and subsequent injection pressure is actually on par for the method average but that is the peak that looks poor.
Also while purging all the channels and lines, I noticed a large leak in the valve interface module crevice that holds the leak sensor. It wasn't a slow drip, but a dump of a good chunk of liquid. This has only happened during the purge and I haven't been able to reproduce the leak. I don't know if it was bc I had the purge valve too loose or if there is a slow leak back there but purging a high volume of leak finally exposed it.
Any help would be appreciated. Our lab is a start up and doesn't have a service contract. We have the guy who set up our lcms installation, but if we don't have to pay for an expensive fix, it'd help a lot.