u/redIIIhead

Hi! I work as a PCR test developer and have run into a problem.
My experience isn't sufficient to resolve it, so I’m posting here. Background signals have started appearing in my system. When using a primer-to-probe ratio of 3:2.5:3, I get weak curves (up to 50 RFU), yet the test system fails to detect samples with concentrations below 1,000 copies/mL.

We order our primers and probes from GenScript. Can anyone help?