u/redditnessdude

Hi, dual-citizen of Ireland and the US here. My entire adult life I have wanted to move back to the EU, but I'm hesitant to completely jump ship especially because I've spent so much time specifically laser focused on US medical school requirements. Also, from what I understand my MCAT isn't transferrable to an Irish medical school because it's too old.

I was wondering what it would be like if I decided to get my medical degree here in the US, and then later move back to Ireland. I would imagine I have to go through the entire equivalent of residency, what exactly is that process like? How long is it? How likely is it for a nonresident citizen to get into one of these positions? Is the pay just as shoddy as it is here in the states?

Another important thing, does the USMLE (US medical licensing exams) have any value in Ireland?

Hi,

I'm developing an LC-MS assay for tenofovir diphosphate, which is structurally similar to ATP. I am new to chromatography and I've been having a lot of trouble with this compound. Today I discovered that peak shape and intensity significantly degrades at temperatures above 30 C, and actually improved at 20 C.

This is very counterintuitive to me; I would expect higher temperatures to result in a tighter peak and a lower retention time but I'm seeing a broader peak and HIGHER retention time. Does anyone have an explanation for this sort of behaviour? I considered thermal degradation, but I feel like these temperatures are quite low for that to occur especially when the compound is in the column for less than a minute.

My conditions are as follows

Mobile phase A: 15 mM ammonium bicarbonate in water, pH 9

Mobile phase B: 15 mM ammonium bicarbonate in 90% ACN, pH 9

Column: Premier Z-HILIC 2.1 x 50 mm 2.5 um.

Hi, I'm working on an assay for a triphosphated compound, and I'm trying to get down to an LLOQ of 0.1 ng/mL. Problem is that it's a pretty tricky analyte I'm hitting a wall at like 0.5-1 ng/mL.

Currently I'm using an atlantis premier BEH Z-HILIC 2.1 x 50 mm 2.5 um column. There is a 1.7 um version available, and I was wondering what one might expect with a smaller particle size? There is only one compound, so separation is not one of my concerns. Would the smaller particle size be expected to have any effect on the intensity of my peak if I scale my gradient accordingly? Trying to figure out if it's worth looking into.