Hello hello everyone! ✨ I (25F) have been here 2 months now and haven't been able to make a lot of friends to go out (or stay in) and do things with - so I thought I'd try my luck here!
I love going on hikes, trying new cuisines, playing board games and going artsy stuff. I also love singing and would be very down for a karaoke sesh 🎤
Oh and I'm a big fan of pub quizzes if anyone's recruiting 👀
Hello fellow labrats! I tried to wade through the archives to find some help (like I often do when I have issues in the lab 😂) but couldn't find anything so I'm reaching out.
We're trying to make fixed brain slices (fixed in 4% PFA) and typically we use the Leica VT1200 but ours is currently not working (needs a bit of servicing) so we thought we'd try the Microm HM 650 V that we also have in the lab.
The issue is, even after messing around with all possible settings we're having an issue with our tissue slices (as seen in the image below). We've narrowed it down to a mechanical issue but can't make out what exactly is going on. Any suggestions or ideas?