u/stressedglizzy

Image 1 — trying to isolate mycobiont from a sample of lichen, but is this something else?
Image 2 — trying to isolate mycobiont from a sample of lichen, but is this something else?
Image 3 — trying to isolate mycobiont from a sample of lichen, but is this something else?

trying to isolate mycobiont from a sample of lichen, but is this something else?

I’ve been trying to isolate a lichen mycobiont from a sample of Xanthoria parietina by an ascospore discharge method. the hyphae exploded from a plate of spores, so I did an agar transfer yesterday and they are already growing rapidly. anyone know if this is mould that I’ve transferred over instead? the morphology looks really similar to the lichen ascospores under the microscope which is throwing me! any advice would be greatly appreciated
pic 1: agar transfer plate after 1 night of incubation. pic 2: agar transfer plate under microscope. pic 3: original ascospore discharge plate with hyphae and spores showing similar morphology

u/stressedglizzy — 1 day ago