Hey everyone, I need some help finding a protocol to isolate lactobacillus acidophilus from yogurt or dairy products so if anyone could help I’ll appreciate it
Trying to finish a chart to be able to complete a dichotomous key for my second unknown assignment for micro class.
There are 12 possible microorganisms and each bench got to test on 3-4. We were told to take pictures of our resulted plates and tubes so we could use that info to fill out the chart.
Unfortunately... Not everyone took clear or good quality pictures so it's hard to tell what I'm looking at or some are labeled poorly.
Is there any reliable online source that I can use to find my answers? I've got everything I could from Bergey's, my lab atlas, and some NIH articles but I'm at a standstill now.
Hi y’all. Attached is a video under 40X light microscopy, of an outdoor microalgal pond, . The water salinity is brackish. I notice this grazer today. Can someone help ID this.
We have published a preprint titled “Evolutionary shifts in spike glycan-binding specificity suggest a possible association with host adaptation during SARS-CoV-2 Omicron evolution” .
I'm having trouble figuring out how to properly do the different staining. Here's how I performed the different stains and my results in an image. The bacteria is Mycobacterium Smegmatis
Gram Staining
Flood slide with Crystal Violet for 1 minute, water rinse
Flood slide with Iodine (mordant), 1 minute, water rinse
Acetone Alcohol drop by drop at an angle until color stops running for 3-5 seconds, water rinse
Counterstain flood slide with Safranin, 1 minute, water rinse, blot dry
Acid Fast Stain
Flood slide with Carbolfuschin for 10 minutes (cold method), water rinse
Apply 5-6 drops of alcohol acid on slide at angle until no more color runs off, water rinse
Counterstain with Methylene Blue for 1 minute, water rinse, blot dry bulbous paper
Endospore Stain
Flood slide with Malachite Green and steam with blowtorch method for 5 minutes (ensure fumes and stain doesn't dry out), water rinse both sides until no more Malachite
Counterstain with 0.5% Safranin for 1 minute, water rinse, blot dry
Problem
The problem I seem to have is for Acid Fast Stain (A), whether I flood the slide with alcohol acid or slowly do it drop by drop at an angle, I always seem to get rid of all the carbolfuschin and I just see the methylene blue under the microscope. The B picture is one that my classmate got for the same bacteria. My staining (A) makes it difficult to identify the bacteria because it looks blue instead of the pink/purple to identify the Mycobacterium.
Endospore, I seem to get spots of green and the rest is pink, which is confusing since this bacteria doesn't have endospores. I should only see pink here confirming the Endospore neg.
Any tips or advice?? Thank you for taking the time to help and read my post :)
These are some of my photos of fungi and slime moulds. I realise many of them aren't that great, still wouldn't hurt to share them. You may recognise some of these from Wikipedia, which is where I used some of them to brighten up biological articles. Feel free to use them for any kind of purposes. Not exactly pure microbiology (sorry).
For my thesis in microbiology II I picked the topic above as my field of interest. I found that the foreskin has so many benefits from an immunological perspective. My paper is peer reviewed by an MD named Dr Mary Zhang MD, she was my professor and used to be a Chinese cardiologist.
Hey everyone, looking for some troubleshooting advice or sanity checks from fellow micro nerds.
We are consistently getting swarming motility with Bacillus spizizenii (<100 CFU / 0.1 mL) on our in-house manufactured Tryptic Soy Agar (TSA) plates.
We are seeing this happen on both spread plates and pour plates. In a typical 4-plate set, they are all the same organism, but the leftmost plate completely loses control and swarms across the surface.
Info to rule out the usual suspects:
Moisture: The plates do not have excess condensation or visible droplets.
Srain: We are locked into this specific strain for regulatory/testing requirements.
Occurancss: This is happening across multiple scientists, different media batches, and different organism sources (both commercially purchased vials and our own frozen cryovials).
Any tweaks or recommendations would be appreciated!
How do you take sample out of it, what if coli bacteria isnt there where you take it but in sample it self. Do you blend it all together or sum. Prolly bad question i just was thinking this other day lol.