u/SpiriRoam

r/DIYbio r/microbiology r/Agarporn

Image 1 — Daily Bacteria Isolation #31🦠White Wrinkly Swarmy, Gram-(probably gram+ but stained gram- anyway), nonmotile, catalase-, oxidase-, grown and isolated from soil on NA. Daily isolation discontinuing probably until august because of family vacation in a week. It will be worth the wait.

Image 2 — Daily Bacteria Isolation #31🦠White Wrinkly Swarmy, Gram-(probably gram+ but stained gram- anyway), nonmotile, catalase-, oxidase-, grown and isolated from soil on NA. Daily isolation discontinuing probably until august because of family vacation in a week. It will be worth the wait.

Image 3 — Daily Bacteria Isolation #31🦠White Wrinkly Swarmy, Gram-(probably gram+ but stained gram- anyway), nonmotile, catalase-, oxidase-, grown and isolated from soil on NA. Daily isolation discontinuing probably until august because of family vacation in a week. It will be worth the wait.

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Daily Bacteria Isolation #31🦠White Wrinkly Swarmy, Gram-(probably gram+ but stained gram- anyway), nonmotile, catalase-, oxidase-, grown and isolated from soil on NA. Daily isolation discontinuing probably until august because of family vacation in a week. It will be worth the wait.

u/SpiriRoam — 13 hours ago

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Daily Bacteria Isolation #30🦠Transparent, Gram-, motile, catalase+, oxidase+, grown and isolated on NA at 28C from pond scum. TSI slant incubating. Planning to make urease test medium tomorrow. Cant do indole till after July 18th when my kovacs reagent arrives. Video separate post.

u/SpiriRoam — 2 days ago

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Daily Bacteria Isolation #29🦠Purple Streptomyces, Gram+, nonmotile, filamentous, catalase+, oxidase-, grown on ISP2 agar at 28C isolated from soil on SCA 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video in comments.

u/SpiriRoam — 3 days ago

▲ 4 r/Agarporn+1 crossposts

Daily Bacteria Isolation #28🦠Multi-isolation Special Agar Collage day, Black(because of the media), grown and isolated on Brain Heart Infusion Agar and broth with Potassium tellurite 140 mg/L 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video of all the behind the scenes in comments.

u/SpiriRoam — 3 days ago

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Daily Bacteria Isolation #27🦠Yellow, nonmotile, gram-, catalase-, oxidase-, grown and isolated on NA from pond scum, tsi slant had no growth

u/SpiriRoam — 5 days ago

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Daily Bacteria Isolation #26🦠Yellow, nonmotile, gram-, catalase+, oxidase-, grown and isolated on NA from pond scum, tsi slant had no growth(still unsure why im also questioning the stain). Video in comments.

u/SpiriRoam — 6 days ago

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Daily Bacteria Isolation #25🦠Lime Green, nonmotile, gram+, catalase-, oxidase-,no endospores, grown on ISP2 Agar at 28C isolated from dirt on SCA 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video in comments.

u/SpiriRoam — 7 days ago

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Daily Bacteria Isolation #24🦠Tan Yellow, nonmotile, gram+, catalase-, oxidase-,no endospores, grown on nutrient agar at 28C isolated from dirt on SCA 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video in comments.

u/SpiriRoam — 8 days ago

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Daily Bacteria Isolation #23🦠Clear, Gram Negative, motile, catalase+, oxidase+, full red tsi slant. Grown and isolated on Nutrient agar at 28C from pond scum. Video in comments.

u/SpiriRoam — 9 days ago

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Daily Bacteria Isolation #22🦠White Swarmy, Gram Negative, nonmotile, catalase-, oxidase-, completely yellow tsi slant. Grown and isolated on Nutrient agar at 28C from pond scum. Video in comments.

u/SpiriRoam — 10 days ago

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Daily Bacteria Isolation #21🦠Lime Yellow, nonmotile, catalase+, no endospores, grown on nutrient agar at 28C isolated from pond scum on SCA 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video in comments.

u/SpiriRoam — 11 days ago

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Daily Bacteria Isolation #20🦠Brown Streptomyces, Gram Positive, nonmotile, filamentous, catalase-, grown on nutrient agar at 28C isolated from pond scum on SCA 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Photos in profile.

u/SpiriRoam — 12 days ago

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Daily Bacteria Isolation #20🦠Brown Streptomyces, Gram Positive, nonmotile, filamentous, catalase-, grown on nutrient agar at 28C isolated from pond scum on SCA 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video in profile.

u/SpiriRoam — 12 days ago

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Daily Bacteria Isolation #19🦠White Mucus, Gram Negative, nonmotile, catalase-, oxidase+ Grown and isolated on nutrient agar at 28C from pond scum. Video in separate post. TSI slant incubating for days 11, 18, and 19.

u/SpiriRoam — 13 days ago

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Daily Bacteria Isolation #17🦠Pink, Gram Negative, probably motile, catalase+, grown and isolated on nutrient agar at 28C from pond scum. Video in profile

u/SpiriRoam — 14 days ago

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Any knowledge on what makes bacteria decide to swarm a plate or not?

First plate is it stabbed it center and second plate is iso streaked. This is under 24 hours of incubation at 30C. Heres examples of others doing the same thing but not quite as drastic.

u/SpiriRoam — 14 days ago

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Daily Bacteria Isolation #17🦠White Streptomyces, Gram Positive, nonmotile, filamentous, catalase+, grown on nutrient agar at 28C isolated from pond scum on starch casein agar with 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video in separate post in profile.

u/SpiriRoam — 15 days ago

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Daily Bacteria Isolation #16🦠Golden White, Gram Negative, motile, catalase-, grown and isolated on nutrient agar at 28C from pond scum. Video in separate post in profile.

u/SpiriRoam — 16 days ago

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Daily Bacteria Isolation #15🦠 White Spiral Swarming, Gram Positive, nonmotile, catalase-, grown and isolated on nutrient agar at 28C from pond scum. Inoculated via one jab in the center. Video posted to profile.

u/SpiriRoam — 17 days ago

▲ 0 r/microbiology

Daily Bacteria Isolation #14🦠White, Gram Positive, cocci?. nonmotile, catalase-, grown and isolated on nutrient agar at 28C from dirt. Photos in separate post.

u/SpiriRoam — 18 days ago