u/SpiriRoam

I am asking this because I can’t really think of much right now, so I’m probably just gonna end up doing soil again since I had the most fun with that. I’m preparing to do the daily isolation again. After many weeks of work, I made fresh ISP2A plates, NA plates, SCA plates, CGXII, CGXIIA plates, Christensen’s urease agar plates, MacConkey agar plates, TSI slants, NB, BPW + glycerol, and PBS. I’m also going to make BG11A and BG11 and SIM medium in a couple days, and then I’ll start streaking. I’m just waiting on my ferrous ammonium sulfate and sodium nitrate to arrive for it. I can also make BHI and BHIA and stuff with Tween 80 and 20 in it. I also have Instant Ocean with germanic acid for some diatom-free marine culturing if anyone suggests that.
I just inoculated some SCA with soil again to start up my actinomycetes train again. Funnily enough, I think I was making it wrong for all those isolations in the past. I used casein enzymatic hydrolysate instead of undigested casein. This time I used some micellar casein I had on hand. I hope it’s more selective even with the colistin, nalidixic acid, and cycloheximide I put in it.

Any sample suggestions for the daily isolation?(no skin flora or fusobacteria places)

Daily Bacteria Isolation #31🦠White Wrinkly Swarmy, Gram-(probably gram+ but stained gram- anyway), nonmotile, catalase-, oxidase-, grown and isolated from soil on NA. Daily isolation discontinuing probably until august because of family vacation in a week. It will be worth the wait.

Daily Bacteria Isolation #30🦠Transparent, Gram-, motile, catalase+, oxidase+, grown and isolated on NA at 28C from pond scum. TSI slant incubating. Planning to make urease test medium tomorrow. Cant do indole till after July 18th when my kovacs reagent arrives. Video separate post.

Daily Bacteria Isolation #29🦠Purple Streptomyces, Gram+, nonmotile, filamentous, catalase+, oxidase-, grown on ISP2 agar at 28C isolated from soil on SCA 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video in comments.

Daily Bacteria Isolation #28🦠Multi-isolation Special Agar Collage day, Black(because of the media), grown and isolated on Brain Heart Infusion Agar and broth with Potassium tellurite 140 mg/L 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video of all the behind the scenes in comments.

Daily Bacteria Isolation #27🦠Yellow, nonmotile, gram-, catalase-, oxidase-, grown and isolated on NA from pond scum, tsi slant had no growth

Daily Bacteria Isolation #26🦠Yellow, nonmotile, gram-, catalase+, oxidase-, grown and isolated on NA from pond scum, tsi slant had no growth(still unsure why im also questioning the stain). Video in comments.

Daily Bacteria Isolation #25🦠Lime Green, nonmotile, gram+, catalase-, oxidase-,no endospores, grown on ISP2 Agar at 28C isolated from dirt on SCA 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video in comments.

Daily Bacteria Isolation #24🦠Tan Yellow, nonmotile, gram+, catalase-, oxidase-,no endospores, grown on nutrient agar at 28C isolated from dirt on SCA 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video in comments.

Daily Bacteria Isolation #23🦠Clear, Gram Negative, motile, catalase+, oxidase+, full red tsi slant. Grown and isolated on Nutrient agar at 28C from pond scum. Video in comments.

Daily Bacteria Isolation #22🦠White Swarmy, Gram Negative, nonmotile, catalase-, oxidase-, completely yellow tsi slant. Grown and isolated on Nutrient agar at 28C from pond scum. Video in comments.

Daily Bacteria Isolation #21🦠Lime Yellow, nonmotile, catalase+, no endospores, grown on nutrient agar at 28C isolated from pond scum on SCA 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video in comments.

Daily Bacteria Isolation #20🦠Brown Streptomyces, Gram Positive, nonmotile, filamentous, catalase-, grown on nutrient agar at 28C isolated from pond scum on SCA 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Photos in profile.

Daily Bacteria Isolation #20🦠Brown Streptomyces, Gram Positive, nonmotile, filamentous, catalase-, grown on nutrient agar at 28C isolated from pond scum on SCA 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video in profile.

Daily Bacteria Isolation #19🦠White Mucus, Gram Negative, nonmotile, catalase-, oxidase+ Grown and isolated on nutrient agar at 28C from pond scum. Video in separate post. TSI slant incubating for days 11, 18, and 19.

Daily Bacteria Isolation #17🦠Pink, Gram Negative, probably motile, catalase+, grown and isolated on nutrient agar at 28C from pond scum. Video in profile

Any knowledge on what makes bacteria decide to swarm a plate or not?

Daily Bacteria Isolation #17🦠White Streptomyces, Gram Positive, nonmotile, filamentous, catalase+, grown on nutrient agar at 28C isolated from pond scum on starch casein agar with 127.5mg/L cycloheximide, 40mg/L nalidixic acid. Video in separate post in profile.

Daily Bacteria Isolation #16🦠Golden White, Gram Negative, motile, catalase-, grown and isolated on nutrient agar at 28C from pond scum. Video in separate post in profile.

Daily Bacteria Isolation #15🦠 White Spiral Swarming, Gram Positive, nonmotile, catalase-, grown and isolated on nutrient agar at 28C from pond scum. Inoculated via one jab in the center. Video posted to profile.