First DMT clone bioassay of the F2 hybrids
Following the transplanting of 750 F2 hybrids and 10 North African accessions, a preliminary bioassay was conducted on two high-yield DMT clones. These clones were selected from the hybrid plot based on Thin Layer Chromatography (TLC) analysis, specifically targeting non-methoxylated alkaloids.
- Material & Extraction: The plant material consisted of 8.5g of fresh leaves, which were subsequently dried to a final weight of 2.000g as determined by a 0.001g moisture analyzer. The extraction method involved boiling the dried leaf material twice in distilled water with citric acid, each for 30 minutes. The resulting solution was then basified with NaOH and extracted twice with chloroform. The chloroform pulls were combined and evaporated in a pre-weighed 50mL beaker. The crude extract yield was 18mg from 2g of dry leaf, representing a 0.9% crude yield (w/w dry weight). Based on dose-response, the crude extract was estimated to be approximately 50% DMT, resulting in a net DMT yield of approximately 0.3–0.4% of dry leaf.
Pic 5, 6 is the TLC analysis result of one of the two clones tested. Clone sample is lane 7. Lane 4 is MHRB. All lanes on the plate are 25mg dry weight samples soaked in 1ml chloroform + few drops of 25% ammonia. Notice the two largest blue fluorescing spots at the top (DMT RF) are the MHRB and the DMT clone (which based on it's DMT RF fluorecence intensity seems to be roughly 40% that of MHRB DMT spot, which is backed by the practical crude yeild extracted.
Pic 3 and 4 are the selected clones being transplanted for clonal propagation.
Pic 5 is the 8.5g fresh leaves extracted which gave the 18mg crude freebase.
Bioassay Results: The bioassay revealed that the onset, peak, duration, and subjective effects were strikingly similar to that of DMT extracted from MHRB.
To be absolutely sure i pulled out my Stash of MHRB extracted DMT freebase and vaporized some.. it only lead to increased intensity of the same effects i had from the hybrid clones extract. I wish i smoked the clone extract at higher dose all at once... The room was just starting to morph when i reached the peak of the last dose and had no more left to smoke.
I was smoking the. Rude freebase in incremental doses to explore it. Now that i am familiar with these selected clones benign qualities my next harvest i will go for breakthrough.