r/NTNPerformance

Could the RS pin the above near the injury if stacked into same vial? NTN state twice a day for wolverine but once on KPV so thought id ask before they mixed them.

GHK-Cu is one of the most searched compounds in the space, mostly for skin and hair research, and it works differently from every other skin ingredient it gets compared to. It's also one of the few here with real dermatology data behind it. Research framing throughout, so here's the rundown.

What sets it apart from retinoids and vitamin C is the target. Those push tissue to produce more collagen. GHK-Cu doesn't. It signals tissue to organize the collagen already present. That distinction matters, because aging skin isn't only short on collagen, the collagen it has is disorganized, microcirculation is reduced, and cells sit in a defense state rather than a repair one. GHK-Cu shifts that balance. Microarray work shows it moving expression across more than 4,000 genes, pulling cells out of damage response and into organized repair. The reported result is tissue that reads structurally firm rather than temporarily plumped.

Part of why it behaves this way is that it isn't foreign. GHK is a tripeptide the body produces on its own for tissue maintenance, and plasma levels fall roughly 60% between age 20 and 60. That decline parallels the age-related drop in wound healing capacity, which is why it's studied as a compound that restores a signal lost over time rather than one that introduces something new.

There are two routes in the research, and they reach different depths. Topical formulations reach the epidermis and shallow dermis, the layer relevant to fine lines, texture, and the surface of scar tissue, and topical is typically applied daily or nightly. Injectable reaches the deeper dermis and soft tissue, the layer relevant to structural firmness, lax zones, and thicker scars, and injectable protocols run 2 to 3 times weekly rather than daily. The cheat sheet reflects that split. The reasoning given for the lower injectable frequency is a narrow signaling window, where the remodeling phase happens between applications and constant stimulation can bias tissue back toward inflammation.

On reconstitution, 4 mL of bacteriostatic water into the 100 mg vial yields 25 mg/mL, so 1 mg is 0.04 mL, 2 mg is 0.08 mL, and 2.5 mg is 0.1 mL. A 50 mg vial takes 2 mL for the same concentration. The reconstituted solution turns blue from the copper complex, which is the expected appearance and not a sign of anything wrong. Application is subcutaneous into the deeper dermis, not intramuscular, and a regional grid pattern around a target area is used rather than a single site. Topical research concentrations run 0.1 to 0.3% for facial work and 0.5 to 2% for scar or scalp work over 8 to 12 week courses, with hair timelines closer to 3 to 6 months.

A substrate note that comes up throughout the research: GHK-Cu functions as an organizing signal, and a signal can't build matrix without raw material. Adequate protein supplies the collagen building blocks, vitamin C serves as a cofactor, and stable sleep supports the repair environment. Protocols that ignore substrate tend to underperform regardless of dose.

On timelines, the observed sequence is texture and hydration shifts by weeks 2 to 4, fine line softening by weeks 4 to 8, and structural firming and mature scar changes by weeks 8 to 12. Hair endpoints run longer at 3 to 6 months and pair better with minoxidil or microneedling than as a standalone. On evidence, GHK-Cu sits on firmer ground than most cosmetic peptides, supported by gene expression data, decades of study, and its status as an endogenous compound, which is a stronger footing than the mouse only data behind much of the sheet.

Contraindications worth noting in any protocol: copper handling disorders such as Wilson's disease, since the compound adds copper load, active malignancy in the treatment area, and pregnancy, where safety data is absent. Total copper load should be accounted for when other copper containing products are in use.

GHK-Cu remains a research compound and everything above is research context only.

curious how people are approaching GHK-Cu in their research, injectable or topical, and what the timeline looked like

Full doses and the topical breakdown are in the pinned cheat sheet: https://www.reddit.com/r/NTNPerformance/comments/1tht5o3/the_only_peptide_cheat_sheet_youll_need_doses/

Thoughts on each? Experiences?

40M , Started researching MOTS-C . Titrated slowly up from 1mg to 4mg bi-weekly. I didn't feel much initially. Strength workouts actually got worse. Somewhere around week 2-3 . I started to notice my cardio sessions getting effortless. I used to struggle running 1-2kms at a stretch. Now, for someone who doesn't really enjoy running , I managed to run of 7kms continuous at a decent place. Which was around week 4 of MOTS-C.

I see the benefits and want to include it as part of a long term regimen.

One concern I have is downregulation of endogenous MOTS-C production with longer term usage.

Any researchers who've used multiple cycles of MOTS-C? Multiple months, years? What were your experiences ?

By chance is it possible to combine these? Just curious.

heyo,

i was curious what is the difference between these. I tried researching it on here and most posts are 11 years old. So i figured i would ask again for perhaps better answers.

anyone who used different kinds and compared? intranasal vs subq injections differ per or are all the same?

so far i read that each one is more potent than the other and that adalank/adamax are the strongest. But some people felt nothing with adalank/max nor na /na amidate.

thanks.

Has anyone had experience with using bpc157 and ghk-cu to help with recovery.

For staying VERY lean what's a worth it stack.
There's so many exercise mimetics like SLUPP, AOD, MOTS C, 5AMINO and appetite suppresers like GLP1s and cag and then there’s visceral fat burners like tesa+ipa, if i want to hit almost every path way possible what's the most worth it way to do that?

Hello everyone,

I have one 3ml BAC vial and 2 15 mg vials, so adding 1 ml to 15mg vial, means I have enough BAC water for the two vials?

1. Good results from 1mg a day, 5 on 2 off?

or should rat be given 2 mg

2. I am about to order 10 vials from another suppier much cheaper but US based. so that would give me 50 more shots for my RAT.

3. The vials from original suplier have COa with > 99%, enotxcins within range.

4. Do you test every vial.

5. What is worst case scenario if vial has impurities?

Thank you,

Jimmy

Bonjour à tous,

​Je suis un protocole sous tirzépatide (Retatrutide) depuis quatre mois et j'observe une amélioration significative quant à ma perte de masse grasse. En parallèle, je m'entraîne au gym à raison de cinq séances par semaine.

​Cependant, je constate une baisse importante de ma force athlétique. Souhaitant éviter le recours aux stéroïdes anabolisants, je m'intéresse aux alternatives du côté des peptides (tels que le CJC-1295, le MOTS-c, le Tesamorelin ou encore l'hormone de croissance), mais il est difficile de faire un choix éclairé parmi l'éventail des produits disponibles.

​Selon vos expériences, en particulier pour ceux qui maintiennent un rythme d'entraînement similaire de cinq jours par semaine, quelle approche ou combinaison s'est avérée la plus efficace pour préserver la force ?

​Merci pour vos retours et vos conseils.

14 days ago i opened a Brand New 10ml bac water vial and used 2ml bac water on my mt2.

After i puncutured the bac water vial i put it back in my shelf in room temperature.

Today i used the same bac water vial for my retatrutide. Should i have had put the bac water in the fridge after i punctured it for the first time?

FYI the reta vial i used the bac water with today looks completely clear with no floaters.

Am i good to go or did i fuck up my New reta vial by using the bac water i opened 14 days ago and had it stored in room temperature?

FYI on my bac water vial it clearly says Store At RT.

Epitalon is the one people run for longevity and telomeres, and it also has the widest gap in this whole space between what's genuinely proven and what gets claimed about it. The mechanism is real and now independently confirmed. The dramatic lifespan numbers are not. Both of those are true at the same time, so here's the honest rundown.

It's a tiny four amino acid peptide built off an old bovine pineal extract, developed by the Russian gerontologist Vladimir Khavinson. It goes after two aging mechanisms at once: telomere shortening, by switching on telomerase, and circadian breakdown, by restoring your pineal melatonin production. That second one is why the first thing people notice is sleep, usually better sleep quality inside the first week or two. Set expectations here though, you won't see or feel anything dramatic, no skin or hair transformation. Better sleep is the near term signal that it's doing something, and the rest is a long bet.

The interesting part is how it works, and why the dosing looks so strange. Epitalon is small enough to slip into the cell nucleus and bind DNA right at the telomerase gene, turning up hTERT, the catalytic engine of telomerase. In cell studies that produced real telomere lengthening, around a third longer in cultured human cells. And the dose response runs backwards from what you'd expect. It works best at extremely low concentrations, and piling on more does nothing. That's the tell that it's an epigenetic signal, it flips a switch that stays flipped rather than a drug you keep topped up. Which is exactly why you run it in short courses instead of daily. Ten to twenty days triggers changes that last months.

Here's the part to be straight about, because it's where this compound lives or dies. The mechanism is real and, as of 2025, confirmed outside Russia for the first time, a Brunel University study reproduced the telomerase activation in human cells. That matters, because for 40 years nearly all the research came from Khavinson's own institute. What has not been confirmed is the big stuff, the famous longevity figures like a four fold drop in mortality. Those come from that single group, they've never been replicated, and the institute holds close to 200 patents on this class, so there's real commercial motivation sitting behind the claims. So the honest position is simple: the cellular mechanism is proven, the human life extension is not. Run it knowing you're making a mechanism based bet, not following settled science.

Both the 10 day and 20 day options add up to the same 100 mg per cycle, so pick whichever you'd rather run. Dose it in the evening, 1 to 2 hours before bed, because it works through your pineal and melatonin rhythm and morning dosing throws that timing away. For the draw, mix the 10 mg vial with 1 mL of bac water for 10 mg/mL, so 10 mg is 1 mL and 5 mg is 0.5 mL. That 1 mL is a biggish subcutaneous (SC) shot, which is part of why a lot of people run the 5 mg for 20 days version instead, since it halves the nightly volume. Most people do 2 to 4 cycles a year, and going higher doesn't help, it was tested up to 50 mg a day with no added benefit, which fits the low concentration mechanism.

One thing to address head on, since it always comes up. Switching on telomerase sounds alarming, because telomerase is exactly what lets cancer cells become immortal. Fair concern. The wrinkle from that 2025 study is that Epitalon seems to do the opposite in cancer cells, inhibiting telomerase there and pushing a different pathway, which may be why animal studies saw fewer tumors despite the telomerase activation elsewhere. That's genuinely promising, but it hasn't been validated in humans, so the rule holds: do not run it with active or suspected cancer. Same for pregnancy, breastfeeding, and known immune issues, since none of that has safety data behind it.

Side effects are mild in practice, some injection site reactions, occasional fatigue or drowsiness that passes in a few days, and sometimes vivid dreams from the melatonin and REM effect, which most people take as a good sign. The bigger caveat is at the system level: it's FDA Category 2 as of 2024, so it can't be legally compounded here, it's research only, and a 2025 review flagged that the core safety data, long term effects, immunogenicity, drug interactions, simply hasn't been studied properly. So the near term tolerability looks fine and the long term picture is honestly unknown.

if you've run Epitalon, did your sleep change in the first couple weeks, since that's supposed to be the tell, or did you run it purely on the telomere theory and feel nothing. curious which camp people land in

Full doses and bloodwork are in the pinned cheat sheet: https://www.reddit.com/r/NTNPerformance/comments/1tht5o3/the_only_peptide_cheat_sheet_youll_need_doses/

Hi. I’ve been on Reta for three weeks and had next to no side effects and good results, appetite suppression has been mild though. The one side I have got is pretty awful unfortunately (skin pain/sensitivity) my blankets even touching my legs at night hurts a lot.
Therefore I’m thinking of switch to Tirz.

Im wondering how I go about this? My current Reta dose is 3mg a week.

Do I have to have a break before switching to tirz? Can I just go to Tirz when I’m due my next Reta dose?

What dose do I start on tirz if I’ve already been taking Reta for a month?

Thanks for any advice.

Hey everyone! I am new to peptides and wanted some guidance on stacking. This is purely for research purposes.

Currently I take:
Reta - 20 units once a week
KLOW - 10 units 5 days on 2 off
NAD+ - 10 units daily

First, is that a good dosage point. There’s so much info out there.

Also, I’m wanting to add Tesamorelin/Ipamorelin Blend and wanting to get some knowledge on dosing for that. It’s a 15mg vial before reconstituted.

Thanks!

I’m reading there are two types. Wondering what experience you’re RS has had with these if you’ve used both over time?

1. Standard bacteriostatic water — sterile water with 0.9% benzyl alcohol added as a preservative (this is what “BAC water” defaults to, and what your current BA10 supply is). The benzyl alcohol prevents bacterial growth across multiple draws from the same vial, which is why it’s used instead of plain sterile water. But it is not isotonic — its saline/osmotic profile doesn’t match your body’s tissue fluid.
2. Isotonic (0.9% saline) bacteriostatic water — same benzyl alcohol preservative, but formulated with 0.9% sodium chloride so the osmotic pressure matches your blood/tissue fluid. This is the one that reduces injection-site irritation — because the solution’s saline concentration matches what’s naturally in your tissue, there’s no osmotic gradient causing local stinging, burning, or swelling at the injection site. Plain BAC water, by contrast, can sting or irritate on its own simply because it’s not isotonic — independent of whatever peptide is dissolved in it.

Anyone have advice on dosage and protocol for Reta, NAD+, KLOW and Tesamorelin/Ipamorelin Blend?