u/Brief_Awareness_8231

Hi everyone

I am doing my first bacterial transformation and was hoping to get some advice.

1. The lab post doc said I could concentrate my bacteria before plating (spin down, remove half SOC and resuspend in remaining) to make sure I get some colonies. Since I was worried about my efficiency doing this the first time that's what I did. However, when I came in to get the plate after incubation, the colonies were a lot smaller and clustered than I was expecting. While there might be a couple isolated colonies I could try and pick, there does seem to be satellite colonies nearby. I still have more of my transformed bacteria in the fridge and wondering if I should dilute and plate again to try and get less dense colonies for picking?

2. Our lab tech poured some new agar plates but accidently forgot to put antibiotics in them, I have read your can spread antibiotics over these; just wondering if anyone has done this and can recommend what concentration of Amp to use?

Thank you!

https://preview.redd.it/5kt32v6fr1bh1.jpg?width=2183&format=pjpg&auto=webp&s=551a6939d0c05915565f80522f94b282ea329cec

Hi all,

I am unfortunately late on filing my taxes this year because of some family health crises.

I am a little unsure of what province I should file taxes in this year. I am born and raised in Alberta, out of province for my PhD in Ontario and I have always filed in Alberta because of my student status. But this year, my boyfriend and I bought a condo together in ON.

Does my student status mean I should/can still file in AB? Or do I now need to file in ON? And if I change my province of residence for taxes do I need to change it for my driver's license, health card, and student loan?

Thank you

Hi all

I submitted samples for proteomics for the first time and I got my results back. I get both raw data, but also the log2FC and p-adj value.

So now I have thousands of DEP that I am not entirely sure what to do with. I know a couple people in my lab have mentioned string and gprofiler, but I am wondering if there are other tools (free) that I could use to either pull top hits and meaningful pathways out of this. Thank you!

Hi everyone

I have a small west-facing bedroom with a large window in my top floor condo, which despite central AC still gets quite hot. I am thinking about applying a heat control film just to help maintain the temperature a bit.

I know there has been posts on here about this before but I am getting a bit confused. I have been looking at the Gila products specifically (titanium or 3-in-1). I have read that if you use these with low-e coating you can be at risk of the window cracking. But then on the Gila instructions it says this is for the older style of low-e coating, which mine wouldn't be as it is a new build.

I am just wondering if anyone can help clear up my confusion as to whether either of these films would be safe for my window or if there is a better product thank you!

Hey everyone
I hope this is the right place to ask this. My condo has central AC and all the rooms in the house are good temperature wise with the exception of one. There is a smaller bedroom, that only has one AC vent and a large west-facing window. Despite AC this room gets hot. I am wondering if anyone can recommend the best option I can try to help keep it cooler. I’ve seen heat protectant window treatment, small portable AC units, dehumidifiers, and booster fans and I could really use some advice.

Thank you!

Hey everyone

So I have been having quite an unlucky time with my glasses the past couple months. I had a pair of WooW glasses for about 3 years, I absolutely loved them - but the hinge broke and the arm fell off. I was told by my optical place it couldn't be repaired so I glued it in the meantime while I looked. Then a family member was diagnosed with cancer so I had to fly across the country to help out, I was the primary driver at this point so I was really worried about my broken glasses not holding up, but I also did not have the capacity to really go on the hunt and wait for new glasses.

So there was this little business, a kiosk in the mall which cut your lenses in 30 minutes, had very cheap frames (online for 60$) which I ended up getting to hold me over, and thought I would keep them as my backup pair. One of the little nose pad arms ended up busting off of these before I had even gotten a chance to order a real pair.

I have never had so many glasses breaking in such a short time, I am feeling just a bit flustered? Is 3 years for WooW frames a good amount of time for metal frames? Do you get better quality with more expensive frames? Because I have a very narrow bridge I can almost never find acetate frames which fit me, not sure if these would be more durable and worth really searching for?

Any advice appreciated thank you!

Hey all
I am in the second year of my PhD and have lately just been really feeling like I’m really in a slump. My MSc project was histology and mouse behaviour but now I am working with iPSC models with goal of doing molecular assays. I have picked up the cell work well even the differentiations. But I feel embarrassingly behind in molecular techniques. I am only starting to learn western blot, PCR, and omics. I feel super slow learning these too - I read about them, watch tutorial videos and then feel so awkward when doing them. The ones I have been doing with a bit of help from the post doc haven’t completely failed but I feel this immense anxiety that I should already have mastered these.

I also have this sense of dread that I will never catch up in these techniques and will never get to a place I am confident in performing them. Any advice would really be appreciated!

Hey everyone
I keep hearing how durable titanium frames are supposed to be. I’m currently shopping for new frames and would love to get something durable. I saw a pair I really liked from Titanium Optix and was just wondering if they are really worth the hype?

Thanks!

Hey everyone

My brother (23M) was diagnosed with B-ALL almost a month ago now. When I got the call from my mom about his diagnosis, I put everything on hold to travel across country to help. I am a PhD student in biomedical sciences; stopped my experiments, and postponed my candidacy exam. In the first week and a half of my arrival my brother was very sick; couldn't stand, nausea, and sleeping most of the day. Thankfully he really started to improve once they started his chemotherapy. He started telling us he was feeling better than he had in 6 months, eating a lot, going for daily walks, planning his courses for undergrad, and making mathematical formulas to better play his video games. During this time, I was the designated person to get any meals my brother requested to supplement his hospital meals; pretty much everyday I would get whatever he requested to bring to him. I also managed chores at home because my mother is mentally ill, and cannot manage doing both visits to the hospital and regular daily tasks. I would also have to wake her up otherwise she would sleep till 4PM, then I would drive her to the hospital and back. I was also the mediator of any squabbles between my parents, them with my brother, or them with other family members. It was pretty exhausting.

I had been there for 3 weeks so I decided to book my flight back as the doctors were really happy with his progress, and I needed to return to my studies. I told him my brother a week before my flight that I had to return, and the only thing he said was "oh no, who will bring me my ice cream now". But a day before I was supposed to head back he had to be transferred to the ICU because of an infection in his central line. My mom was an emotional disaster, so I had to take her to the waiting room to calm her down, my dad was at home and didn't know what to do. My mom really wanted me to stay, and brought it up in front of my brother that she thinks I should stay longer until he's out of ICU. Now I understand he is in the ICU and probably frightened with what has just happened but he was still feeling pretty good. He replied to my mom and said "okay" and shrugged his shoulders, then went back to talking about his favorite engineering and physics principles.

Wake up the next day, and I am not feeling very well. My mom wanted to sleep at the hospital because it was my brother's first night in ICU, so I called her and the first thing she says to me is that he wants a specific Italian dish brought for him in a couple hours. I told her I couldn't come at all today because I was feeling unwell.

I am now having these very strong mixed emotions. I am at a point where I feel like I've reach my limit here, and I want to return to my PhD. I am starting to feel irritated by my brother's demands without any sort of "thank you". And then both of those thoughts make me feel guilty, because I understand he has cancer. Not sure if there's any advice to give but I just needed to vent a bit. Thank you!

Hello

I have a second generation AirPods from a few years ago that I was going to give to my mom to have. They had been unused for maybe 8 months so I went to charge them and test them to see if they are still working well. The left AirPod is fine, but I have noticed the right AirPod is not detected as out of the case when I remove it, and is then not detected in the ear either. The AirPod shows it’s charging when in the case and out of the case.

I’ve done a few resets on the AirPods but the problem still remains. Should I try cleaning the case, is there anything else to try? Thank you

Hi everyone

I recently got new frames and lenses, but I’ve been having massive problems adjusting. They are metal frames and it seems that no matter how much I adjust them I am massive pain, and pressure on my bridge. Additionally I am having a very intense headache mainly concentrated on my forehead area. I can’t figure out if this is normal in adjusting to new glasses or if something more is wrong? The headache is progressing to where I’m almost vomiting.

Any advice would be appreciated! Thanks

Hey everyone

A few years ago, I got my WOOW Day Off 2 frames. I really love these frames but I guess they’d gotten worn and the arm snapped off. The store I got them from said they were discontinued and couldn’t be repaired or replaced. Since I take a long time to find frames, I decided to glue the arm on to use in the meantime. Then I had a major family emergency come up and had to fly out of province. I went to a few optometry places while in town and one of them was selling my exact frames and offered to put my lenses in them. I decided this was a good option with all that was going on.

Unfortunately I’ve been wearing these frames for a little over a week and they just don’t seem to be sitting right. I’ve been getting a headache and muscle strain around my eye. I’ve had multiple adjustments, even went to my mom’s optician who didn’t like the angle of the glasses but did make the best improvement with her adjustments.

Is it possible that the same frames from different places could fit so different? Should I keep getting them adjusted or just return them and start fresh?