u/ExpensiveNight5790

Image 1 — [contamination] is my liquid culture contaminated or just over-colonized?
Image 2 — [contamination] is my liquid culture contaminated or just over-colonized?
Image 3 — [contamination] is my liquid culture contaminated or just over-colonized?

[contamination] is my liquid culture contaminated or just over-colonized?

I have this liquid culture jar (cubensis LC) for 2 months or less now. 10 days ago I used half of it to colonize oats and the oats look fine, seems like there is no contam.

But suddenly my liquid culture looks completely different. I haven't checked it in a week and there are really clear white spots inside the liquid culture and on the surface.

I'm guessing it's not contaminated but it's over-colonized. The mycelium blob in the jar grew bigger and bigger until now, there is no more fluid to colonize.

Do you really think it's too dense mycelium or could it be contaminated?

I never stored it in the fridge.

Btw, test it on agar will be the easiest way to know, but I tried agar and it seems like a SAB is not enough in my house.

u/ExpensiveNight5790 — 1 day ago

[actives] is my monotub too wet

I spawned to bulk (jack frost) my first time on may 3th. On may 10th, the whole tub was colonized. I think the fast colonization is because I let my grain spawn over-colonize a bit I think.

Once everything was colonized, I noticed water dripping down the sides onto the substrate forming little puddles. It messed with the mycelium on those places (it just went coir-brown again on the bottom of the puddles). So I dried the walls of the monotub for 2 days. There was way less condensation after this, and the water wasn't dripping down the walls anymore.

Now, today, I noticed there is suddenly a lot of condensation again and the pattern the tub dries (pastywhyte's ez dialed monotub) seems really odd. This is the first time the tub dries like this (first picture).

The substrate has beautiful water pearls, just like it supposed to be I think? (Picture 2)

The other pictures are showing the date. You can see the tub being too wet I think.

Should I increase fae and drill some more holes? Or leave it like this?

i think the drying pattern is also really strange. What about that?

u/ExpensiveNight5790 — 7 days ago

Hi everyone, I did my first G2G transfer last week (cubensis, Jack Frost) and everything seemed well. This morning I decided to do a shake and break because the jars were colonizing really well and were colonized about 30-40%. (Picture 4)

Now, 5 hours later, I went to check the jars and the grains seemed blue/green, definitely a color I haven't seen in my jars before. In my opinion it definitely seems contaminated, but I think it's weird this color wasn't present before the shake and break. The mycelium was growing well but it was hard to break it up in the jar (I thought maybe because the transfered grains were over-colonized). As far as I know the mycelium won't bruise blue like the mushrooms do?

All my jars now have this strange color. Is this contamination or should I wait a bit longer before jumping into conclusions?

The pictures are showing the weird spot, the last picture was the jar this morning before breaking the mycelium.

u/ExpensiveNight5790 — 16 days ago

Beginner here, I had 1cc of LC and no jars anymore so I thought, why not use the last cc to expand it.

I never made LC before so I just messed around a bit, I used 1/4 grain boiling water, 3/4 water and 1,8% honey.

In just 4 days the liquid culture expanded from a small blob to a jar full of mycelium, I'm really happy with the results. The color cleared up (last pic is after PCing) and the mycelium expansion is crazy.

The strain is MAK95 (can't really find a lot about it).

I honestly didn't think it would expand this fast, and I have no idea what to do with it. I have 20 jars going at the moment and this is all for personal use/friends, so maybe I should just put it in the fridge to save for later?

u/ExpensiveNight5790 — 23 days ago
▲ 1 r/MushroomGrowers+1 crossposts

I'm a beginner and this is my 2nd time making bulk substrate, the coir I bought in the same store is full of fibers. The first time it was really fine ground coir, almost like peat. Should I buy a new brick of coir or will this be ok?

First picture is the new fibrous coir, the second picture is the 'regular' one

Edit: I'm growing cubensis

u/ExpensiveNight5790 — 24 days ago