Outside of backstory, what is the worst day of your OC's life?
A lot of characters are initially motivated by some tragedy in their younger days that continually pushes them forward. However, I'm more interested in the moments after their initial motivation that weigh on them as the worst moments of their life.
For Tainaka Nao, it would be the death of Sir Nighteye. When she was 8, her parents both died in front of her because of a building collapse. She couldn't use her biokinesis quirk to heal them, because as an 8 year old, she didn't know enough about biology to fix what was broken.
But with Nighteye, she knew exactly what needed to be done. She barely knew the man, but after the Shie Hassaikai raid, she was the best option for healing him, and she knew what she needed to do.
The issue was that he had so much going wrong in his body she couldn't keep up. She had to force his heart to keep beating, replace the lost blood in his body, keep his organs free of internal bleeding, help his lungs take in enough oxygen, and between all that, somehow find time to actually heal him. It ends up all being too much for her that she was falling behind and he was getting worse even through her healing.
So he still ends up dying, and it's all while she's actively healing him and getting a constant awareness of his entire body as he dies. It's worse for her now because she isn't limited by the knowledge she possessed like with her parents, but she still couldn't save him because she just wasn't good enough.