How to extract DNA from fecal samples stored in liquid nitrogen?

Hi everyone,

I need to extract DNA from cecal/fecal samples stored in liquid nitrogen using the QIAamp PowerFecal Pro DNA Kit, which requires up to 250 mg of sample. What is the best way to handle the frozen samples to weigh 250 mg while minimising thawing? Any recommended protocols or practical tips would be greatly appreciated.
Thanks!

reddit.com
u/Ok-Air9195 — 2 days ago

How to extract DNA from fecal samples stored in liquid nitrogen?

Hi everyone,

I need to extract DNA from cecal/fecal samples stored in liquid nitrogen using the QIAamp PowerFecal Pro DNA Kit, which requires up to 250 mg of sample. What is the best way to handle the frozen samples to weigh 250 mg while minimising thawing? Any recommended protocols or practical tips would be greatly appreciated.
Thanks!

reddit.com
u/Ok-Air9195 — 11 days ago
▲ 2 r/DNA

How to extract DNA from fecal samples stored in liquid nitrogen?

Hi everyone,

I need to extract DNA from cecal/fecal samples stored in liquid nitrogen using the QIAamp PowerFecal Pro DNA Kit, which requires up to 250 mg of sample. What is the best way to handle the frozen samples to weigh 250 mg while minimising thawing? Any recommended protocols or practical tips would be greatly appreciated.
Thanks!

reddit.com
u/Ok-Air9195 — 12 days ago