r/DNA

▲ 25 r/DNA+1 crossposts

Hair strand analysis- where should I send it?

I have a braided strand of hair in a bible from the 1800’s. I’m pretty sure I know at minimum the family the hairs belong to…

I am wanting to send it somewhere to get it analyzed. I know that might be a big ask. I have reached out to bode technology and gene by gene, as they were recommended through my research. Family search may do it- but I’m thinking I probably should call and verify/talk it over.

Does anyone have any experience doing this? How did you go about it, and what did you think?

Thank you

reddit.com
u/talesofadaughter — 1 day ago
▲ 3 r/DNA

Large cM difference for a match between myself and my sister.

To settle the first question I predict -- yes she is my full sister. We share 2,567cM of DNA and all our known DNA matches.

I have an unknown DNA match in my top 20 closest matches that I share 175cM which Ancestry indicates could be some type of second cousin.

However, my sister only shares about one fifth of this at 39cM with this match which Ancestry places two generations father back.

What, if anything, could possibly be determined about this match and were he might fit in our pedigree tree. He is a person of interest as it seems likely that he is related to us through one of our unknown great-great grandfathers. Both parents of our maternal grandmother were born to unwed, single mothers in the late 1890s on the West Virginia and Ohio border. After decades of genealogy, no one I know has been able to identify either man.

Unfortunately, few of our shared matches in common with this 175/39 cM matches have much in the way of developed trees. I have struggled to make much headway in figuring out how we could be related. It doesn't help that we appear to quickly end up with Irish immigrants in the mid to late 19th century with all the repeating and common names, both first and last.

reddit.com
u/GaelicJohn_PreTanner — 2 days ago
▲ 1 r/DNA+1 crossposts

Company that offers long read WGS?

Is there any company that offers long read Whole Genome Sequencing to consumers?

I have short read WGS from sequencing.com
While that's good for HG38 BAM, it's insufficient for 100% coverage on Y chromosome for T2T BAM, which is what I need to upload to Yfull.com

So is there any company that offers it?

u/Burner-4519 — 3 days ago
▲ 2 r/DNA

How can I find my living relatives using my DNA?

Are there any websites that can help me? I'm aware of 23andme and others but is it possible to find my living relatives (cousins etc) even if they dont know about those websites and never sent their DNA?

reddit.com
u/Far_Information6229 — 3 days ago
▲ 1 r/DNA

Is 10 cM of shared DNA enough for an accurate match?

Had a test done at Ancestry. Using Thrulines, they are showing me 6th cousins with connections through a supposed ancestor. I can go back six generations with confidence, yet Ancestry is showing me two connections through an individual who never existed before I did a DNA test (the individual could be a far flung connection, but certainly not a son of my 4x great grandfather).

reddit.com
u/RedditUser240211 — 5 days ago
▲ 1 r/DNA+1 crossposts

cfDNA extraction in house

Does anyone know a protocol that uses an in-house method to extract cfDNA? I'm trying to do WGS of ctDNA

reddit.com
u/Outside_Compote3066 — 6 days ago
▲ 13 r/DNA

Finding DNA for Indigenous Heritage

Without doxing myself and equally my family. My great grandma lived on a reservation alongside her family before marrying a Black man (paternal paternal) and this is where the story gets sticky-no one knows how they moved. My Dad talked about how she always talked about the reservations, etc but refused to teach her kids anything about the language (assimilation). Her children had actually been labeled as colored which is documented in my family but everything predating her marriage is not documented in her family. They say it’s not uncommon for families to loose connections like this.

My paternal grandma comes from a history of immigrants with some that intermixed with indigenous people and equally with another non white culture.

I actually have family all over due to this and it’s quite interesting the range of skin colors.
My mom’s side of the family actually has documents dating back to the reservations. And I know for mom side the intermixing was a survival which after being displaced it was just a game of survival. Since my great great grandma was also an enslaved indigenous woman the children she had with almost all of them being non consensual. My grandma (great) never met her dad but she knew he was a biracial man (not Black and white but something else). She then moved her kids eventually and came to the state I’m in. Since her children had different ranges of skin color and hair types she thought it would be better than constantly have their safety jeopardized.

Because of this my great grandma continued to pass down traditions from her mom onto well the family. She was always open about our heritage.

My family actually wants to get reconnected but we lost a lot of things due to a house fire.
My thing is while I know the myth of being Black and Indigenous is extremely prevalent. I do wonder how can we get back fully connected with our roots.

reddit.com
u/Minute_Nerve_8985 — 12 days ago
▲ 5 r/DNA+1 crossposts

I thought of a cubing idea

I just thought of an idea of cubing, and it’s not what you think. The idea i thought of is a website where cubers can take a DNA test and get the results of cubing. Have you ever had that time where you thought you can’t get any faster? ya, i had. But i want to help cubers. The secret of getting faster is in your DNA. And the website’s name is CubersDNA. CubersDNA is 23andMe for cubers. And when you get the results, you get your TPS potential, your lookahead potential, and your efficency potential, there is also a schedule of the ideal day of life to get faster, including improtant but out of cubing stuff like sleep, diet, and exercise. So, you can just buy a kit and spit into a tube and mail it back to scientists and after some time, you get your results. And since you’re DNA don’t change, you don’t have to take the test again. You can enter your average for further accuracy. To be clear, this website isn’t a thing when I posted it, and i don’t make websites. It’s just my idea, but i believe some cubers can make the idea to reality. Ya, the logo should be a blue strip and an yellow strip, and a orage stripe, with a DNA on top of it. You might think that the idea is crazy, but there are athlets taking DNA tests to get better, and there are studies on DNA of chess players, so why not cubing? Are any sub-10 cubers willing to make a website for that? Then, what are you waiting for? Let’s go!

reddit.com
u/Sea_Orchid223 — 10 days ago
▲ 1 r/DNA+2 crossposts

I received a "universal compatibility" text file from sequencing.com which had 500,000 plus minus entries like this one:

rs1801133 1 11856378 GG

The files has this info as well:

For each SNP, we provide its identifier (an rsid or an internal id), its location on the reference human genome, and the genotype call oriented with respect to the plus strand on the human reference sequence. We are using reference human assembly build 37 (also known as Annotation Release 104).

The issue is that this file lists: GG for rs1801133 chromosome 1 position 11856378. This does not align with results i get when i look at the raw data files like the bam file.. (both hg38 and t2t). or fastq files..

Here is what AI seems to conclude:

Final Audit Summary

You have officially completed a "Deep Dive" audit of your MTHFR gene.

  • The Report: Said "Normal (GG)".
  • The Raw Data (BAM/FASTQ): Every test we ran (T2T, HG19, HG38, and FASTQ grep) showed you have the A allele (Mutant).
  • The Verdict: You are Homozygous Mutant (TT) for MTHFR C677T.

The Final Breakdown

  • Location11796312 (The hg38 site for MTHFR C677T).
  • Reference ColumnA
  • Your Data Column,,,,.....,,,,,,,,,..,,.,,,.., (All dots and commas).

 The "Aha!" Discovery

This explains why your DNA report and your BAM seemed to disagree. In the specific hg38 FASTA you are using, the "Reference" base is already an A.

  1. Standard hg38: Usually has a G at this spot.
  2. Your FASTA (assembly38): Has an A (the mutant allele) as the reference.
  3. Your DNA: Also has an A.
  4. The Result: Because you match your specific reference, IGV and Samtools show it as "Normal" (Grey/Dots). The Real-World Verdict

Even though you "match" this specific reference file, an A at this position in the human genome is the C677T mutation.

  • Your GenotypeHomozygous Mutant (TT).
  • The DNA Report: If the report said GG, it was likely trying to say "You match the reference," but it used the wrong letter (G) for a reference that is actually an A, or it used a completely different assembly logic.

 Final Audit Results

You can now confidently record your MTHFR status based on your raw 30x WGS data:

  • rs1801133 (C677T)Homozygous Mutant (TT) (Confirmed by 29 reads).
  • rs1801131 (A1298C)Normal (AA) (Confirmed by your previous check of 31 reads matching the T reference).
u/JamezBond007 — 14 days ago
▲ 2 r/DNA

Why pellets didn’t comeout but like this?

Why pellets didn’t comeout but like this?

u/Personal-Sale-4844 — 10 days ago