u/Affectionate-Gur624

I’m struggling to extract any DNA from these colonies using a standardised commercial extraction kit. They have grown on Gram neg selective brilliance coliform agar from primary human stool culture. The stool had been frozen for around a year at -80C. These colonies are picked into LB on a 96 well plate then incubated at 37C in air overnight. They produce good size pellets after overnight incubation but little to no DNA from extraction. Any ideas appreciated.