u/CorgiLizard

Fungal/mycelial hyphae following static/kinetic forces external to the agar.

Second generation run/isolation of wild southeastern US Panaeolus. Cyanescens on active charcoal-LMEA.

Wanted opinions on this transfer of liquid culture from Panaeolus cyanescens. Most plates look like the first photo 7/10 to be exact, but three have bacterial blotching, one more so than the others (see last photo). Toss LC and started over with Agar-Agar transfer? Try LC with BRF cakes?

Context is quite clear with the caption, but a ~9m old Pan. Cyan plate I had on LMEA w/ charcoal somehow hadn’t dried out on the shelf. Took this and multiple cultivate cubensis plates to revive via liquid culture and tear on agar, low and behold ~48hrs later and we have life!

(Ignore splotches on agar — deposited spore solution from liquid culture.)

This is a peptone dextrose with light malt extract formulation at about 2% sugar. It was growing beautifully and I recently noticed a bit of what seemed like mycelium possibly attached to and growing from the glass. Contamination or a mycelial rafts caught on caramelized sugar?

LC has stayed clear and small spots look non-flat to the surface and alike to the floating media. Panaeolus Cyanescens agar wedge transfer