uni is a joke. where are all the “leftists” i was promised by conservative media????
as someone who is also neurodivergent & UW + nutritionally deficient just outright say you don’t care about the animals, i’ll have more respect for you then…
hey rats, i’ve been doing something that i just realized may not be acceptable. i have been skipping my lunch hour and instead leaving work an hour early. i know it’s not the best practice, but i have horrible anxiety which makes eating in any space besides my home extremely difficult and nerve-wracking. i really thought nothing of this until recently, when i heard a couple of co-workers gossiping about my schedule.
i know my pi is very flexible with how i allocate my hours so i don’t think he would find my schedule unacceptable, but i would like some opinions from people here. is this “okay” to do? what potential issues could this cause? thanks!
Drought, extreme heat, and heatwaves are the most prevalent trio of hazards endangering millions of children globally, according to new climate report.
hello, i'm working with a novel fusion protein. it's highly aggregation-prone, and right now it's in 8M urea-containing buffer. When diluting it stepwise from 8-->4-->2M, the protein remains mostly soluble. however, i'm having issues maintaining solubility when dropping from 2 to 1M. i'm diluting my buffers very slowly (over the course of an hour with constant mixing in the cold), but the proteins still are crashing out. My buffer components are: 50mM tris-HCL, 100mM KCl, 10% glycerol, pH 8 (PI of protein is 9.7). i've tried adding arginine and increasing the salt concentration, but my proteins still crash out. if anyone could help, that would be greatly appreciated. thank you!