WESTERN BLOTSSS HELP
hi!!! I just started a research position which involves a lot of western blots and while other members in the lab have tried explaining this to me I just can’t get the hang of orientation.
- so in my lab they used the same volume of ladder on both sides of the sample
- do you all load the samples with the buffer dam facing you and the gel facing away (and do left to right) or the samples face you and load it right to left???
- and then how does this orientation change on the membrane, will the samples still be in the same orientation or will it flip???
- I’m really losing it cause I can’t figure it out but I’d really appreciate any help at all!!!! or any tips or tricks that have helped people figure it out
- someone suggested that I load one side with more ladder than the other but I don’t know how well that will work.
- PLSSSS HELP ME!!