Reason for my poor peyote yields was not grafting.
So I didn’t have a control on this experiment but a year or two back I found out that GA3 causes near zero mescaline yields on cacti and have had this result twice and already knew to avoid it for alkaloid increasing.
Originally tested a high GA3 foliar to try and induce flowering to no success. I didn’t use the GA3 so didn’t think much about it and with the near zero yield on the peyote grafts I associated it to them being grafted which was a mistake on my end part.
I’ve been experimenting a different PGH in the cytokinin class to induce flowering and didn’t consider the effects on alkaloid content. Last week I did an extraction on my strongest bridgesii at 3% hcl content and to test an alcohol only extraction path and had zero precipitations so I decided to add another precipitating agent and only had 2 crystals form which proved it was indeed poor yield and not the technique that failed.
I started trying to figure out what happened and researched more deeply the PGH and saw that this and pretty much most PGH auxins and cytokinins increase GA3 internally. And this is almost certainly the reason I had near zero yield in my lophophora extraction since I apply my experiments to my entire collection to help collect more data quickly.
GA3 kills yield in higher doses in cacti. Maybe lower doses won’t matter and other plants may have different effects but with mescaline it makes it near zero.