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r/microscopy

▲ 10 r/microscopy

Computational frame differential video- live-view best watch full screen on computer

Using normal light field, opening in python OpenCV - live-view USB industrial camera, converting the input to gray-scale, adding green for frame differences. Sample from my brew of Bristle Moss developing well, seem like an Ark for Noah for micro-wild life. objective 10x

u/LostInGermany4297 — 3 hours ago
▲ 3 r/microscopy

Does it actually make sense to normalize fluorescence intensities to fluorescent beads (e.g. TetraSpeck)?

I have heard that some people normalize the fluorescent signal of their samples to the signal of fluorescent beads (eg. TetraSpeck) imaged either on the same slide or on a separate slide, and I'm struggling to understand the logic. First, I;ve heard of it but never saw anyone actually doing it.

The beads were never treated the same way as the sample. They weren't fixed, permeabilized, blocked, stained with antibodies, or subjected to the same mounting/clearing conditions. So how can their signal say anything meaningful about variability in my sample? Considering I'm always using the same settings in the microscope and the microscope is well calibrated.

Also that's another point - in the beads manual it says that these beads are used for calibrating the microscope, not samples. Therefore, my current understanding is that bead normalization is only valid for instrument-side variability — not for sample-side variability (staining efficiency, fixation, antibody batch, autofluorescence, RI mismatch in the sample). Is that right? Are there cases where it's reasonable to use beads beyond that, or am I missing something?

Would appreciate input from anyone who routinely does quantitative fluorescence imaging. Thanks!

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u/EqualNo1154 — 4 hours ago
▲ 2 r/microscopy

What are these?!

Fast moving spinning thingies, video isn't even sped up!

Rain puddle sample

ESAW MM02 compound microscope

300x magnification

u/Enderixius — 5 hours ago
▲ 90 r/microscopy+1 crossposts

Cute little springtail I found in one of my moss samples. He also deleted itself from existence, leaving the boundaries of time and space, I wasn't aware they could do that.

u/Thrawn911 — 17 hours ago
▲ 7 r/microscopy

Euplotes on a DIY microscope

Euplotes are just amazing to see they look like a multicellular organism yet they are not !

DIY microscope (MicroVoyager 🔬) fully hand made by repurposing and modifying parts and lenses from old electronics and cameras.

MicroVoyager 🔬- Infinix SMART 7 phone camera - pond water - total magnification on screen ≈ x200.

u/Ahmadase — 9 hours ago
▲ 1 r/microscopy

microscop equiepment

hi everyone

Hi everyone,

I’m looking for a used Meiji Techno EMZ-200 or similar microsurgical stereo zoom body with around 200mm working distance for a DIY dental microscope project.

I’m especially interested in:

- Trinocular versions

- Body/head only

- Compatible camera ports (C-mount preferred)

If anyone has one for sale or knows where I can find one, please let me know.

Thanks! #microscopy

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u/EkAr1 — 14 hours ago
▲ 1.8k r/microscopy+2 crossposts

I just lost my job, had a terrible day, but I finally managed to find my third ever tardigrade, so it war all worth it

u/Thrawn911 — 1 day ago
▲ 3 r/microscopy+1 crossposts

Labomed vs Optika?

I'm thinking about buying one of these two. Which would you consider better assuming similar characteristics in both models?

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u/DrMarranito — 20 hours ago
▲ 52 r/microscopy+1 crossposts

Paramecium in cell division (Speed up 4x)

Digilab DI-132b microscope, 100x, 1.6x digital zoom, Galaxy A34, Brazil

u/LuizinJegue123 — 1 day ago
▲ 11 r/microscopy

How good of a deal is this?

Hello guys. I saw this Zeiss on marketplace in my city. It costs $250 Canadian dollars. I just got myself a microscope, but this post just can't get out of my mind. I wonder if I can play with it and resell it for a slightly higher price.

The head thought doesn't look like the most heads I saw in google. I wonder if it's an older model or something.

It says that the condition is good. It includes x4, x10, x40 objectives and five positions lens stage.

u/Michael2526 — 24 hours ago
▲ 56 r/microscopy

Pond - Kratella, Fragellaria etc

Made with Zeiss Axioplan 2, 40 an 63, Dik and Lamdafilter, Canon Eos700D

u/ToeKey3947 — 1 day ago
▲ 325 r/microscopy+1 crossposts

Though an onion seems completely inert on the surface, at a microscopic level, it's an entire metropolis with highways and delivery drivers that never take a break.

u/sorin1972 — 2 days ago
▲ 31 r/microscopy

Finally found a ciliate staying still for once

K55-BSA, Polarized light + mica plates, Plan 10/0.25, 20/0.4 and 40x/0.65 objectives, ELP USB Camera

u/Horst-Schopis — 2 days ago
▲ 33 r/microscopy

A Freshwater Copepod

Darkfield photograph of a copepod using a Brunel EyeCam Plus on a Brunel SB40 microscope with a 10X Plan objective. Also tried using Helicon Focus for image stacking.

u/SmallWorldNavigator — 2 days ago