u/AAAAdragon

I understand that you either need to move on or get back together. But when you are demisexual and not over your ex you dodge so many people who are red flags to be in a relationship. Like there are a people who are a good match for you but there are a whole whole lot more people who are attracted to you and who would ruin your life, but when you are not over your ex you reject them. But if you weren’t over your ex, you might make some bad relationship decisions.

So I just crystallized one of proteins to high resolution and the electron density map shows Tris unquestionably bound exactly where the substrate lysine is supposed to bind in the active site.

Tris is a primary amine buffer. Lysine is a primary amine. 100 mM Tris, pH 8.5 likely outcompetes lysine for the active site.

So if I want lysine bound I guess I can’t use amine based buffers. Maybe I should use phosphate buffer … ?

Pretty crazy!

My trainee didn’t notice Tris bound in the 1.6 angstrom resolution structure. Good thing I made a big scene about “Wow! You see every non-hydrogen atom in that electron density map so how do you not see that Tris molecule!?” before we deposited the structure to the protein data bank, LOL.

So I heard about this SARS2-CoV 2 database at a scientific symposium.

Basically, the database is https://coronavirus3d.org/

The whole SARS-CoV-2 RNA genome has been mapped for a while now because of the scientific response to the COVID19 pandemic. The database just seems to show the full RNA genome of the virus, the structure of all the experimentally determined proteins of SARS-CoV-2 and the common mutants over time. For instance, the site shows a graph of the number of observations of certain variants over a narrow time window. Data seems to be missing from March 2025 to present. Though, I am not sure if it is because the archives this database is drawing from stopped collecting data or if this database isn't just up to date. I am not sure.

I attached some graphs of what the database shows for, instance.

https://preview.redd.it/3h5ka1qyj8kh1.png?width=1919&format=png&auto=webp&s=720ab84a02aa8f0e9c4c018bd49c4d7462e53420

https://preview.redd.it/ij7ox3wxi8kh1.png?width=1919&format=png&auto=webp&s=eb372b7225940960fbca8c1e176376d2b0013c27

The main targets of SARS-CoV-2 virus seem to be the Spike protein and nonstructural protein #5 (Main Protease) to my knowledge. I actually work on the Main protease and while the structure is very obviously known the number of inhibitors it can bind seems to be endless based on a search of all of its structures in the PDB.

The website database is still under development. I am not involved with the development. I just think it is very interesting to see all this data in one place. https://coronavirus3d.org is actually a sub-website of https://pathogens3d.org/ .It just so turns out that Coronovirus3D seems to be better put together given the scientific response to the COVID19 pandemic.

My cat Bessie likes to look out the window in the mornings and she has a favorite windowsill in my bedroom. That’s her favorite spot to absorb the sunshine and look at the birds, rabbits, squirrels, and people walking by. So I raise all the curtains in my bedroom

I can also open the curtains in the living room which doesn’t have as good view. If I do that then the sunlight fully fills my apartment.

I just wonder if she likes full sunlight or for the living room to be dark so she can take a nap in the mornings? I have asked her but I don’t speak cat so I’m not sure.

What do your cats like? Full sunlight or partial sunlight?