My son was playing with this clothing shelf and the edge came out… is this something I can fix myself? Like maybe find the nearest two studs and screw in a beam over the corner support to brace it solidly into position??
Somewhat DIY noob here but willing to try!
I've been messing around with the Neon NxT for electroporation, and I generally like it. I was initially worried about the expensive gold-tipped pipettes, but I find that I actually end up going through the plastic cuvettes much faster because so many fewer are provided, and restocking on them is really not cheap! Does anyone reuse them or autoclave them?
Thanks in advance!
Just got some human recombinant interleukin 2 from peprotech, and looks like it needs to be reconstituted initially in Acetic acid... how critical is this? Could I use something else, like hydrochloric acid or just plain dPBS/water? Anyone try anything different?
Just bought the Dynabeads T cell activation beads, and the protocol says to wash them first with the help of a magnet... How important is this step? We do not have the magnet... Anyone try an alternate method of washing and/or skipping washing altogether?
I know this has been asked in the past, but it keeps coming up - which IDE are people using for coding with LLM support these days? Continue/Roo are now dead... Any free / open source options?
I got a plasmid from addgene, streaked it, grew a colony, sequenced it - it was identical to what we ordered. I used two primers from IDT (i checked the sequences - no other internal binding sites possible unless I tolerate 15+ mismatches), after 35 cycles I got 2 bands (the expected is 6kb). Any ideas why this might have happened?
Molecular bio noob here…
I’m testing some guides for CRISPR Cas9 in preparation for an HDR experiment. I used IDT’s HDR design tool to help design guidez, and cross checked the guides using ChopChop.
I’m going to do a quick Cas9 test without template to start to check if the Cas9 even cuts the right spot with these guides, but to verify this I will need to sequence the area. ChopChop has designed 4 primer pairs for this, would I need to try multiple or can I go with one pair alone? None of the pairs have any predicted off-targets and the Tms are all between 59.8 and 60.3
Any thoughts or insights would be most welcome :)
I work with pluripotent stem cells and need to modify some of these lines for a variety of purposes, mostly for targeted Knock ins. I’ve been looking into setting up HDR combined with Cas9, but I’m going crazy trying to figure out how to combine the optimal cut with the optimal homology arms, so the design part is a struggle. Technically we can do all the steps once the design is complete, but I’m worried that even then, if something was off with the design, we’ll spend a lot of time troubleshooting.
So I’m thinking it may be worth just going with a kit or service like TruDesign from Thermo or something from similar from IDT, Takara, etc.
Does anyone here have any service they use to help design/set up HDR experiments? Any testimonials would be greatly appreciated!
I differentiate pluripotent stem cells and track changes in fluorescence over time. The thing is the auto fluorescence properties change as differentiation proceeds (cells get smaller, more complex, etc) making it hard to directly compare signal intensities or even to draw gates that apply broadly across time points. Would using a spectral analyzer help with this?
Thanks in advance!