u/Falanadhamakaaa

Those who got their bfp before missing their periods did you visit you doctor immediatelyor waited for a few days?

Hello everyone
So i got a faint positive on hpt today at 10 dpo. I am not planning to see the doctor until after i have missed my period theoretically.
So considering my previous loss should i start taking progesterone 200mg vaginally (since i have some left from when my doctor last prescribed them) . And my doctor had also suggested previously to take it for the cycles i am trying but I didn’t take any this cycle.

I was transiently transfecting my A549 cells for a co immuno precipation experiment in 60mm dish. I need at least 1mg of total protein lysate. But all I am getting from a 60mm dish is just around 400ug total protein.
My supervisor said that one 60mm dish would be sufficient for recovering 1mg but i am no where close.
Where am i going wrong??

Edited to add:
I perform transfection at approximately 80% confluency. And harvest protein lysate at 48 hours post transfection.
Prepare my lysate using CST (cell signalling lysis buffer) . I add ( PMSF and protease inhibitors) additionally.
Protein concentration is estimated through bradford assay.

Yesterday, some guests came to our home. They have a very cute 6-month-old baby. God bless her.
She was sleeping on my 6-year-old son’s bed, and seeing her sleep there brought out all his emotions. He became adamant about keeping her and making her our little baby. When the guests finally left, he just kept crying and saying that he doesn’t have a baby while all his friends do, and that God never listens to him.
So my question is: during such situations, what do you tell your child to make them feel better? Because saying, “We’ll get one soon,” clearly didn’t work for us.

Hi guys can you all suggest me a good supplement available in India which would help with overall sperm health.
My husband’s Semen analysis came back pretty good, except that his volume is too low (1ml only). My doctor was not concerned as rest of the things were good.
But we think we should try a supplement and see if it helps.

Hello everyone we are trying to conceive for more than a year now. We got our blood work done recently as we are having difficulty to conceive.
I am 30 years old and my reports done on cycle day 2 are as follows.
Vit D- 37.4 ng/ml
Vit B12- 263 pg/ml
HbA1c- 4.8 %
Estimated Average Glucose (eAG)-91.06 mg/dL
Fasting Glucose- 81 mg/dL
Triglycerides- 73 mg/dL
HDL Cholesterol-43 mg/dL
LDL Cholesterol-96 mg/dl
Ferritin- 13.70 ng/mL
Free T3- 3.31 pg/mL
Free T4- 1.25 ng/dL
TSH (Ultra-sensitive)-2.985 µIU/mL
FSH- 5.2 mIU/mL
LH- 3.51 mIU/mL
Prolactin- 16.59 ng/mL
Estradiol (E2)- 36.4 pg/mL
Total Testosterone <12.98 ng/dL
Free Testosterone- 0.98 pg/mL
DHEA-S -104.50 µg/dL
SHBG -54.60 nmol/L
AMH -3.02 ng/mL
Fasting Insulin- 5.81 µU/mL
HOMA-IR- 0.74
Beta Cell Function (%B)- 97.50 %
Insulin Sensitivity (%S)- 135.80 %

My previous cycle progesterone level was - 8.1 ng/ml

My husband who is 38 years old. His semen analysis report is as follows.
Volume-1.0 mL
Sperm concentration- 32.5 million/ml
Total motility- 60%
Progressive motility- 55%
Normal morphology -80%
Vitality (live sperm)- 60%
Fructose- Present
WBC- 2–3/HPF

His semen culture was also fine.

Does our report looks normal or not?
As i am going to see my doctor tomorrow, also suggest me what should I specifically talk to her about.

Hello everyone we are trying to conceive for more than a year now. We got our blood work done recently as we are having difficulty to conceive.
I am 30 years old and my reports done on cycle day 2 are as follows.
Vit D- 37.4 ng/ml
Vit B12- 263 pg/ml
HbA1c- 4.8 %
Estimated Average Glucose (eAG)-91.06 mg/dL
Fasting Glucose- 81 mg/dL
Triglycerides- 73 mg/dL
HDL Cholesterol-43 mg/dL
LDL Cholesterol-96 mg/dl
Ferritin- 13.70 ng/mL
Free T3- 3.31 pg/mL
Free T4- 1.25 ng/dL
TSH (Ultra-sensitive)-2.985 µIU/mL
FSH- 5.2 mIU/mL
LH- 3.51 mIU/mL
Prolactin- 16.59 ng/mL
Estradiol (E2)- 36.4 pg/mL
Total Testosterone <12.98 ng/dL
Free Testosterone- 0.98 pg/mL
DHEA-S -104.50 µg/dL
SHBG -54.60 nmol/L
AMH -3.02 ng/mL
Fasting Insulin- 5.81 µU/mL
HOMA-IR- 0.74
Beta Cell Function (%B)- 97.50 %
Insulin Sensitivity (%S)- 135.80 %

My previous cycle progesterone level was - 8.1 ng/ml

My husband who is 38 years old. His semen analysis report is as follows.
Volume-1.0 mL
Sperm concentration- 32.5 million/ml
Total motility- 60%
Progressive motility- 55%
Normal morphology -80%
Vitality (live sperm)- 60%
Fructose- Present
WBC- 2–3/HPF

His semen culture was also fine.

Does our report looks normal or not?
As i am going to see my doctor tomorrow, also suggest me what should I specifically talk to her about.

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Mention of loss!

I recently got my iron levels tested and they came out to be.
Serum Iron -79 µg/dL
Transferrin Saturation- 27.2%
Ferritin- 13.7 ng/mL
Hemoglobin- 11.6 g/dL

What do you all think about my ferritin levels . Are they too low? As i read a research paper which mentioned that levels below 30ng/ ml were found to be associated with infertility.
So could it be the reason i am having difficulty to conceive? I have been trying to conceive from almost 3 years. During which I had an early miscarriage and a second trimester loss last april. Since then we have bee trying without any success.
Rest other parameters seem to be normal including vit D , vitamin B12 (263 pg/ml) is towards lower side but still within the range.
Has anyone of you have success after correcting the ferritin levels. Kindly help me with your insights.

I am new to posting here. Kindly tell if i need to change anything according to the rules.

Mention of loss:

I recently got my iron levels tested and they came out to be.
Serum Iron -79 µg/dL
Transferrin Saturation- 27.2%
Ferritin- 13.7 ng/mL
Hemoglobin- 11.6 g/dL

What do you all think about my ferritin levels . Are they too low? As i read a research paper which mentioned that levels below 30ng/ ml were found to be associated with infertility.
So could it be the reason i am having difficulty to conceive? I have been trying to conceive from almost 3 years. During which I had an early miscarriage and a second trimester loss last april. Since then we have bee trying without any success.
Rest other parameters seem to be normal including vit D , vitamin B12 (263 pg/ml) is towards lower side but still within the range.
Has anyone of you have success after correcting the ferritin levels. Kindly help me with your insights.

I am new to posting here. Kindly tell if i need to change anything according to the rules.

Yesterday, I checked my cervix because I am TTC and trying to get used to how my cervix feels during different phases of my cycle. As soon as I touched it, some blood came out, and I thought my period had started since it is due in 2–3 days. However, when I checked later, there was no blood at all. I checked my cervix again today to see if it would happen again, and it did, just a small amount of blood which never comes out . Could this be due to cervical polyps?

Hi guys
Since yesterday I have tonnes of watery discharge which looks slightly yellowish when i wipe it on a tissue paper . It is so much that i had to use a panty liner and could literally feel it dripping when i woke up in the morning. There is also a dull ache in my back and pelvic region.
There is no smell though.
I am 9-10 dpo and have tested bfn.

Could this be a sign of bacterial vaginosis?

Hi guys. I recently started trying again after a few months break and decided to get my progesterone level tested. As i had good signs of ovulation this cycle like ewcm and a positive opk on CD13. But 7-8dpo progesterone levels are only 8.17 ng/ml.
What should be my next step?