Image 1 — [actives] This box is on its first flush and doesn't look dry, but it is producing very few mushrooms and in a haphazard manner.
Image 2 — [actives] This box is on its first flush and doesn't look dry, but it is producing very few mushrooms and in a haphazard manner.
Image 3 — [actives] This box is on its first flush and doesn't look dry, but it is producing very few mushrooms and in a haphazard manner.
Image 4 — [actives] This box is on its first flush and doesn't look dry, but it is producing very few mushrooms and in a haphazard manner.

[actives] This box is on its first flush and doesn't look dry, but it is producing very few mushrooms and in a haphazard manner.

Since the mushrooms appeared, I've avoided opening the box entirely, as enough FAE should pass through the lid. There are many newly sprouted mushrooms on the surface (you can't see them clearly in the photo), but will they grow well? Since some mushrooms will be ready in a few days, I'll have to harvest them first and wait for the others to finish growing. I think the problem might be the low humidity (my room temperature is 22.5°C). Do you recommend misting the walls or doing something else to increase the humidity? This is one of the very few boxes that have managed to produce; I want it to perform at its best.

u/moroseta04 — 5 days ago

Can I use food coloring to color the agar? (Ingredients: blue coloring, water, E131 0.4% color, blueberry flavoring). So far, I've always used the agar without coloring it, but I'm having a hard time identifying mycelium growth and contaminants.

I'm experimenting with agar; tomorrow I'll make my fourth attempt. So far, only two plates have mycelium growth, but it doesn't seem to be spreading. All the other plates showed only bacterial growth (I'm using agar to help "clean" my LC of bacteria).

u/moroseta04 — 24 days ago

Second attempt with agar, no growth after a week. The recipe used 5 grams of agar, 5 grams of potato starch, 5 grams of honey, and 250 grams of distilled water. I stirred all the ingredients over low heat to combine, then filled these containers and sterilized them for 50 minutes in a pressure cooke

Both this time and the previous one, I added a few drops of LC to each container, but I didn't notice any growth. What could be the cause?

u/moroseta04 — 1 month ago

Jars of oats that have been sitting for two weeks. The photo shows a jar I opened to see if there were any signs of bacteria.

This jar has the most uncoated parts. Since I'm unsure whether to throw out the other jars, I decided to check this one. When I opened it, I didn't notice any suspicious odor, just the classic mushroom smell. However, there is excess water in the jars and also "mycelial urine." I initially kept these jars between 22 and 26°C, but for a week now I've been keeping them at 27-29°C. Could they be contaminated?

u/moroseta04 — 1 month ago

[actives] How long should I pressure-cook these jars of oats? They hold 200 ml of oats.

All other times, I've filled these jars with 400ml of oats and sterilized them at 14.5 PSI for 2 hours. Now that I've halved the contents, how long does it need to "cook"?

u/moroseta04 — 1 month ago

[actives] 5 jars inoculated with LC AA+. The previously colonized wheat is having difficulty recolonizing after the B&S. Is this normal?

I inoculated these jars on July 27th. But I performed the B&S on different days to see if anything had changed. Colonization started less than 48 hours after inoculation and progressed rapidly. After the B&S, colonization was very rapid, but it's been slowing down for a week. I noticed that the uncolonized parts are the ones that were completely full of mycelium before the B&S. The kernel inside is completely white, but the outside isn't yet. I've had this problem for a long time now and wanted to know if it was normal.

u/moroseta04 — 2 months ago

[technique] Is it possible to prepare petri dishes using the "no-spill" technique and the recipe based on potato starch, agar agra, and honey?

Today I want to try my first attempt with agar. This morning I tried making it, but I think I made a mistake. I put all the ingredients in a glass jar and started shaking it vigorously, but the ingredients separated immediately. From what I understood, I should have mixed the ingredients in a pot on the stove, adding them a little at a time. Do you think doing this would have worked? I'm using a very thick wildflower honey. Otherwise, I found another video where he added the ingredients directly into pressure cooker containers. I managed to get hold of five containers; to "fill" one, you need 50 ml of water. So, based on the recipe I found online, the quantities are: 50 ml water, 1 g agar agar, 1 g potato starch, and 1 g honey. Could I add these ingredients directly to the containers and let them mix in the pressure cooker?

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u/moroseta04 — 2 months ago

For a few weeks now, my room has been a constant 27-30°C (82°F). Is there any chance my box will produce? It's been fruiting for 3-4 weeks, but I haven't noticed any changes.

In addition to this larger box, I have other much smaller boxes that have produced 2-3 small mushrooms per harvest. I have one box that is on its third harvest, but each has produced less than 1 gram of dried mushrooms. Given the heat, the containers were drying out very quickly, but this larger one has LOTS of water droplets on the surface and lid. I'm trying everything I can to lower the temperature in my room, but another extreme heat wave is coming. I keep all the doors and windows open at night, but the air coming in is still very warm, and during the day I seal all the doors and windows.

u/moroseta04 — 3 months ago

box showing 2 different types of contamination, does anyone know what it is?

I made this box by making a big mistake: I didn't mix the oats with the coconut fiber well. Colonization started very quickly at first, but it stopped shortly after. So I started giving FAEs about once a day. For the past few weeks, my room has been around 27-30°C (82°F). [I'm trying everything I can to lower the temperature, but all my boxes are dying now.] In less than 12 hours, a series of white bubbles with many drops of water appeared on the surface of this box. I placed the box under observation, and within another 24 hours, the first bubble turned yellow, and more of these white "bubbles" appeared. Some bright red spots also appeared on some of the oat grains. What could it be? I threw the cake out into the garden to avoid contaminating the whole house.

u/moroseta04 — 3 months ago

Which places should I frequent most to have a better chance of meeting a girl?

Hi, I'm a 22-year-old guy, and my question might seem strange, but I've never been in a relationship. For years now, I've lost the desire to do anything, so anything I do now has to make sense in order to find a girlfriend. Thanks to anyone who can answer!

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u/moroseta04 — 3 months ago

On June 4th, I made this box using a kit box. Two days ago, I removed the lid and put it in the bag, but still no trace of moisture. (I used the same envelope that was provided when purchasing the kit)

When I opened the box, there was a lot of condensation, but in two days there was no sign of moisture on the walls. When I removed the lid, I also noticed some hyphal nodes on one corner, but the situation doesn't seem to be improving. Given the extreme heat where I grow, it averages 28/29°C. And all the boxes I currently have have a lot of condensation on the walls, so much so that they drip onto the surface.

u/moroseta04 — 4 months ago

M 22. Uso tinder da quando ho 18 anni e per ora è stata un immensa delusione.

Leggendo in questo gruppo, ho visto gia molti post di ragazzi che si lamentavano di non ricevere like e non fare macht. In questi 4 LUNGHi anni ho imparato un pò a conoscere tinder e a capire che tipo di persone lo freguentano. Nessuna mi metteva like (a eccezzione di bot o ragazze a più di 400km da me) e questo non mi dava nessuna possibilità di fare "macht". Allora ho iniziato a scrivere su ig a tutte le ragazze che mettevano il proprio ig sulla bio di tinder. Ho notato che è pienissimo di ragazze col proprio ig, ho iniziato a scrivere a ognuna di loro ma il 99,9% o non accettava manco il segui o non rispondevano al primo messaggio. Avrò scritto in questi anni a circa 80-100 profili senza ottenere risultati concreti. Continuando a usare tinder ho notato che ormai i profili erano sempre gli stessi e quindi ho smesso col tempo di usarlo. Circa 2 mesi fà ho letto che tinder usa un algoritmo per farti vedere alle ragazze, e che quindi ormai (dato che nessuna mi metteva like) tinder mi avesse messo come ultima scelta. Allora ho disistallato l app per aspettare 90 giorni in modo da far "resettare" l'algoritmo. Sto attendendo gli ultimi 30 giorni, quindi ancora non so se funziona. Qualcuno di voi ci ha provato in passato? Ha ottenuto dei buoni risultati? Sono disperato, qualsiasi consiglio/idea/esperienza personale è buon accetta. Uniamoci contro quest algoritmo che ci vuole tristi e soli 💪

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u/moroseta04 — 4 months ago

I used an old empty grow kit box to make another grow kit. For the fruiting phase, do you recommend using the bag that came with the grow kit or removing the cake from the box and following the PF Tek method?

I made this box on June 4th using about 250 ml of oats inoculated with AA+. I don't know how well the surface is colonized because I sealed the lid with plastic tape, and the water drops prevent you from seeing underneath. Any advice is welcome.

u/moroseta04 — 4 months ago

I need to buy a new pressure cooker. What features should it have? (Europe)

Here in Europe, it's hard to find pressure cookers that reach 15 psi. The ones imported from abroad are exorbitantly expensive due to shipping costs. I'd like a stainless steel pressure cooker (so it lasts longer) that can reach as close to 15 psi as possible. So far, I've found a very inexpensive 6-liter pressure cooker that reaches 14.5 psi, but since it doesn't have a pressure gauge, I can't really tell what pressure it actually is. I found another model on a website selling a very large pressure cooker from China with a pressure gauge, but it reaches about 13 psi (90 kps) and is made of aluminum (The pot in the last photo costs around €100, much less than other 22-litre pots, so I'm not sure I should trust it too much.). Which one do you think is better? Or should I keep looking? (I can't find anything on Facebook or other used sites.)

u/moroseta04 — 4 months ago

Do any of you use this pressure cooker to sterilize jars? I know it only holds 6 quarts, but the one I had previously held 5 quarts.

I live in Europe and have been looking for a new, reasonably priced pressure cooker for a while. I read in the description of this product that it has a pressure of 1 bar (14.5 psi).

u/moroseta04 — 4 months ago

Is it normal for it to produce fruit so irregularly? The largest mushroom is ready, while other mushrooms are still emerging.

I make almost all the boxes I've ever had this way. I know I can harvest them at different times, but I have to run them through the dryer for 2-3 cycles.

u/moroseta04 — 4 months ago

Are there too many drops of water on the surface? I've left the lid open a bit more in the last few days, but only the sides are drying out.

For a week now, water droplets have been accumulating on the surface. So, to encourage fruiting, I started adding a lot of FAE. This led to the growth of some mushrooms, but many water droplets remained. However, I noticed that the walls and lid are drying out. What do you recommend?

u/moroseta04 — 4 months ago

Years ago, I bought two 1200 ml kits. I kept the empty container and want to try reusing it.

I have a jar of oats that hasn't fully colonized due to issues with the mat (it cooked some oats). Today I was about to throw it away after having it for months, but I noticed that except for the top part and a few grains at the bottom, the rest is completely colonized. I wanted to try scooping out the colonized part with a spoon to use it to make the box. I have two lids to choose from: the first has one of its two filters damaged, and I wanted to know if it would be okay to cover it with a layer of microporous tape to prevent contaminants. The second, however, only has very small holes on the edges, and I wanted to know if they should be covered with microporous tape or left uncovered. Which of the two is better? I haven't found any specific tutorials, so I wanted to know how to do it and if it would work.

u/moroseta04 — 4 months ago

I inoculated these 450 ml jars with 1 ml of AA+ liquid culture on April 22nd. But once they got to 90% colonization they almost stopped.

On May 2, I mixed and broke these three jars. From that day on, growth exploded, bringing them to almost complete colonization in about a week or two. However, once they were almost fully colonized, colonization began to slow down dramatically. Several weeks have passed, and two of these jars were only a few kernels away from being ready. While the third jar (red photo) was still 10-15 kernels away from colonization. So (about three days ago), to eliminate any doubt as to whether it was contaminated, I broke it and mixed it. But where the uncolonized kernels were, a mush remained stuck to the glass. My questions are: are bacteria causing this? Are the first two jars still usable, or is it better to wait for the mycelium to grow further? And why, if the mycelium was able to "beat" the contamination, once I mixed it, did the mycelium completely stop growing?

u/moroseta04 — 4 months ago
▲ 2 r/GroundZeroMycoLab+1 crossposts

I prepared this box on May 8 and put it in the fruiting stage on May 22. Several hyphal nodes have appeared in the last few days. However, I'm concerned about too much water on the lid and the cake.

A few drops from the lid fall onto the cake, leaving some empty spaces (circled in red). Do you think I should try to add more FAE? This will help the moisture evaporate faster.

u/moroseta04 — 5 months ago