u/Murky-Commercial-112

This is only for venting since I am too anxious about my submission. The attorney adre3sed peong 2 RFE in details but kept prong 3 literally unaddressed at all just saying my work is of natural importance even if other employees exist and I have experience and track record. Now it seems like every other one discussed urgency, impracticpality of labor cer etc which is the officer pointed out in their RFE. I feel very sad that I didn't push back hard enough and listened to the lawyer

They said they have a wide firm strategy to keep prong 3 thin, merely saying that the work is beneficial to the USA and the petitioner is experienced even assuming other workers exist, and not responding to the RFE comments on urgency, labor cer impracticality and self employment etc. People who filled RFE with EP, did this happen to you? Did you follow their policy? And did it work out well eventually? Has any of you got denied only on the basis of prong 3?

Prong 3 in our response was basically this:

"In Dhanasar, it was concluded that the petitioner "would benefit the United States even assuming that other qualified U.S. workers are available" due to "his record of successful research in an area that furthers U.S. interests" and therefore satisfied this requirement. The evidence submitted with the original filing and this response also demonstrates that petitioner has considerable experience and expertise in the field and possesses a verifiable track record of research success. Because his record of successful work in this area furthers U.S. interests, petitioner offers contributions of such value that, on balance, he would benefit the United States even assuming that other qualified U.S. workers are available. As such, it would be beneficial to waive the requirement of a job offer and thus of a labor certification."

That's it , no discussion of impracticality of labor cert, urgency, job creation, or the other specific factors the RFE actually listed. When I asked about expanding it, I was told keeping Prong 3 short/streamlined is intentional, since Prong 3 is usually satisfied once Prongs 1–2 are strong, and adding more could invite unnecessary scrutiny.

Has anyone had a Prong 3 this short go through okay, or does this tend to trigger a second RFE/NOID? Trying to gauge how worried to be.

My attorney preferred keeping it thin basically saying that becasue I am well positioned and my work of natural importance to the USA then it would be beneficial to waive the labor certificate even assuming other workers exist. Now the RFE mentioned things like urgency, creating jobs, self employment and harm to USA workers but the lawyer didn't address that at all saying they won't distract from prong 2 and this what worked in the past. Any ideas on that?

Attorney has only sent initial draft a week ago which I edited the same day and sent back now it has been a week since I sent my edits with no response from them and only one week is left for the response deadline, what are the chances the respone could be sent on time? What should I do? And what are my options if the deadline was missed becuae of Attorney delay?

Have a postdoc offer at Harvard and trying to figure iut my finances, what is the cheapest 1 bedroom apartment I can get with bus or train commute up to 45 mins. Also what will be 1 room in shared 2 orn3 room apartment be priced for similar commute?

Thanks

Given how expensive is it to live around Boston area on a postdoc salary, do you think I should accept an offer there?

The offers in Europe came with longer terms, better visa conditions, and more distinguished labs. Still, I chose to continue in the USA after my PhD, where I miscalculated that I would get a green card and make more money in industry. Things turned out to be completely the opposite: my green card NIW application was rejected, my visa is about to expire and wonot be able to visit home soon, and the position is fairly short-term. I'm stuck in a loop of reapplying for a green card, and the string of negative immigration decisions has really gotten to me.

I'm also almost 31, single, in a new city in the USA, with no friends. I have no job prospects in my home country, where science isn't valued or funded the way it is here. I feel stuck in life and don't know what to do.

My attorney has not been responding to my updates regarding the RFE, so I will likely need to finalize and mail the response package myself. I am mainly unsure about whether any additional documents need to be included besides the RFE response brief, supporting exhibits, the RFE notice, and the Premium Processing cover sheet, as well as the correct order in which these documents should be organized. Would anyone be willing to share their package after omitting any perosnal or calssified information?

In addition, the original RFE notice that USCIS mailed to me was accidentally exposed to water, and some of the text has been damaged. I still have the electronic PDF copy of the notice. Is it acceptable to print the PDF version and include that in my response package instead of the damaged original?

I have only a few days left to assemble and mail my EB-2 NIW RFE response package. So far, I have completed the legal brief, organized all of my exhibits, and prepared the premium processing RFE cover sheet.

Before I mail the package, what other documents should I include? Which documents, if any, need to be signed? Since my original Form I-140 petition was prepared and filed by an attorney, but I am preparing and submitting the RFE response myself, are there any additional forms or documents that I need to include (e.g., a new G-28, cover letter, declaration, or anything else)?

If possible, could you also share any USCIS guidance or links to attorneys' recommendations or applicants' experiences regarding self-prepared RFE responses to attorney-filed I-140 petitions? I am on a very tight deadline and want to make sure I do not overlook any required documents.

I interveiwed for the postion and a was asked to send a draft of project idea which I did a week later, then they responded that they will read and get back to me soon. It's already been 10 days now, are they just not interested anymore and when should I follow up again?

Hi everyone,

I want to screen several candidate cytochrome P450 enzymes for conversion to a specific product using transient expression in Nicotiana benthamiana.

I am considering whether several P450 candidates could be pooled in the same infiltration as an initial screen, followed by dropout or deconvolution experiments if product formation is detected.

For anyone who has performed a similar P450 activity screen:

• How many P450 candidates can reasonably be pooled in one infiltration? Can I do 10 together?
• Is it better to test each P450 individually from the beginning?
• How do you keep the total Agrobacterium OD consistent across treatments?

I would appreciate any practical recommendations or published examples.

Looking for side gigs and thought to give this a thought and explore what are legitimiate companies/websites that offer these kinda jobs!

I suck at soccer but really like playing it, but every now and then some competive players will criticize me and make fund of my lack of control and precision, I push back and try to stand my ground but truth is everyone can see I am bad so I lose this fight back. The problem is criticizing make me perform worse. I am 30 yo so can't allow myself to take insults so I stop playing but I really like soccer so I keep coming back, now what should I do keep going and hope for progress or stop to save my dignity?

I'm new to pickleball and looking for pickup games or anyone who'd be interested in playing. Let me know if you'd like to hit the courts!

They asked me to send a rough idea of what I can do in the lab after the intital interveiw. I did but there was no response, i followed up again a week after but no response. Now it's been coupld months, should I follow up again or is it over?

Any thoughts?

I was preparing microsomes from transiently expressed Nicotiana benthamiana leaves for a plant P450 assay,

I also saved some of the crude protein extract before ultracentrifugation (it was centrifuged at 7000 xg to remove cell debris), flash frozen in liquid nitrogen, and stored at −80°C.

I'm wondering whether anyone has successfully detected P450 activity directly from crude leaf extract (or a clarified crude extract after a 7000 × g spin) and or from microsome isolation step.

Any tips or references would be greatly appreciated.