u/daff_7

I’m 22 and recently completed my MSc in Cancer, following a BSc in Medical Biology. I’ve always been passionate about cancer research and medicine, and an MD–PhD really appeals to me because of everything it represents, particularly the opportunity to combine scientific discovery with patient care.
However, the time commitment is making me hesitant. Between the MD–PhD, residency and potentially fellowship, especially if I pursue oncology or surgery, I could be approaching 40 before completing my training.
My biggest concern isn’t necessarily my age, but the experiences I might sacrifice along the way. I want to travel the world, explore different cultures, pursue adventure sports and experience life beyond academia. I’m worried about spending my twenties and thirties constantly working towards the next milestone.
I still have a year before I need to decide, so I’m just trying to understand what life is actually like on this path.
For those pursuing or who’ve completed an MD–PhD, do you feel the sacrifices were worth it? Have you managed to maintain a fulfilling life outside medicine, or do you feel you’ve missed out on certain experiences?
I don’t want to give up on one dream, but I also don’t want to sacrifice all my others to achieve it.

Hi guys! This is a completely hypothetical situation, and I’m quite stupid when it comes to tax, I just want to see how it works.

Let’s say I start a business (would it make a difference if i start as a sole trader or limited company?)
I make my own website and brand eg and sell my products on there. Let’s say hypothetically I make £500k profit from selling, I use £100k to then reinvest to the business (packaging eg)

My ideal goal would be to pay off all my loans and mortgages eg asap, however I am unable to fully grasp the whole tax situation. How do I minimise the amount of tax I have to pay. And would repaying like student loans eg be something consideration? Like I don’t think at all that I can take the remaining 400k and just pay off everything (although that would be a dream) so how does it work. Are there any other sort of caveats?

Also say I have a job aswell, and this is a side hustle that exploded, do I have to take my salary from work into consideration? Or is this where the difference of sole trader and limited company come from?

I also give 10% of each purchase to charity, is this something else I would need to take into consideration?

Sorry if this is silly or stupid, I just genuinely can’t wrap my head around taxes

I swear some people spend the night just to sleep here. Is this allowed? People fully have their legs up snoring and everything

I’ve been commuting for a year, I could’ve done this the whole time?😔

Is there any way to download these?

Hey guys, im new to chess and have taught myself the rules eg and how to play. I am terrible at it, which is to be expected.
I will admit i feel like my reliance on AI during university really stopped me from engaging my brain, and now i lowkey feel like i dont know how to use it
Im unable to see or build strategy when it comes to anything, never mind chess. But specifically within chess how do i build strategy/offend/defend.

I feel like im lacking somewhere

Hey guys! I used to do a lot of climbing and bouldering, however I had a few injuries (car accident) and I had to stop for a while. It’s been a while since i’ve climbed and I have gained around 20kg since I last climbed (puberty weight gain eg breasts, hips, butt) and i’ve been really struggling.
I wouldn’t even say the struggle is in managing to pull myself up but more so my legs. They shake. Like CRAZY. i’ve never had this before. I was told it’s lactic acid build up, and i genuinely don’t know how to stop it and get back to before and calm easy legs

Any tips?

Just noticed there was a new release of tickets today and they didn’t sell out ( in fact there are still loads of tickets left!)
Is the demand over?

Two months of hype and it’s gone

Hi guys, I have a ticket for tomorrow at 14:30pm that I can’t make due to last minute work problems at BFI, London, if anyone would like it please get in touch!

Seat G21!!

Hi! I’m fairly new to FlowJo and wanted to check that I’m approaching this correctly.

I need to identify a specific population defined as:

Marker A⁻ / Marker B⁺ / Marker C⁺ / Marker D⁺

My supervisor has suggested using Boolean gating to find this population, but I’m a little confused about how I should actually set this up in FlowJo, or how to even use boolean.

Would I first gate on the Marker A⁻ population and then look for cells that are simultaneously positive for B, C and D within that population? Or should I create individual gates for all four markers from the same parent population and then combine them using Boolean gating?

If Boolean gating is the best approach, I’d really appreciate an explanation of how to set this up in FlowJo. Alternatively, is there a simpler/more intuitive way of identifying this exact population?

Thanks!

Salam, hope you’re all well!
I’m planning on doing a swimming with orcas excursion at some point, and I’ve been quite particular about the company I would go with because I’d only want to do it with a group that genuinely prioritises the animals’ welfare i.e. doesn’t chase, feed, touch, corner or otherwise disturb them, and allows any interaction to happen on the animals’ terms.
While researching it, though, I’ve come across quite a few people who argue that swimming with orcas is inherently unethical, regardless of how responsible the operator is. I’ve also seen the broader argument that wildlife tourism itself can be unethical because we’re essentially entering animals’ habitats for our own enjoyment , with things like safaris, whale watching, diving excursions etc. sometimes being described as humans “trespassing” on nature.
I completely understand the criticism when tourism disrupts animals, changes their natural behaviour, damages habitats or prioritises getting tourists a good experience over the welfare of the wildlife. The rise in wildlife tourism has obviously created plenty of examples of that.
But I’m wondering whether the activity itself is necessarily wrong if it’s done responsibly. If you choose a reputable operator that maintains appropriate distances, limits numbers, follows conservation guidance and doesn’t manipulate or interfere with the animals, is simply entering their environment and observing them still ethically problematic?
I’m particularly interested in this from an Islamic perspective. Islam repeatedly encourages us to travel through the earth, observe the natural world and reflect upon Allah’s creation. At the same time, we’re entrusted with the earth and surely have a responsibility not to cause unnecessary harm to animals or the environment.
So where is the line between experiencing and appreciating Allah’s creation and intruding upon or exploiting it for our own enjoyment?
Would something like an ethical wildlife excursion be a beautiful way of witnessing and appreciating creation, or should some parts of nature simply be left undisturbed regardless of how careful we try to be?
I’d especially appreciate any answers from a Shia perspective, or references to Qur’an, hadith or scholarly opinions on our responsibilities towards wildlife and the natural world.

Hi, sorry to bother.

I’m doing a flow with both extracellular and intracelullar markers and just confused what to add to each well, especially after fixing and perming

Let’s say i have 3 EC markers, and 3 IC markers, a full stain, no stain, and live/dead.

I go in with a live dead stain on all the wells (minus NS and l/d), incubate and all that

Then i make an EC stain - when i do this, my EC FMO’s will include all the markers (including IC (?)), and my cocktail will include only my EC markers (?), also i will add this to my IC wells too.
Incubate and stuff again

Then i fix and perm

For my IC stain - my IC FMO’s will include ONLY IC markers (minus one), and my IC cocktail will also include only IC markers (?).
Do i add this cocktail to my EC FMO wells aswell?

Aimed for post grads mainly, at student centre or science library?

Hi guys!

I have a ticket to the odyssey BFI on Wednesday 26th August, 2:30pm, and seat G21

I’m looking to swap for any of the midnight screenings, preferably a seat that is above E, and in the middle too

If anyone would like to swap I would really appreciate it!!

Salam guys,

Hope you all are well

Little bit of context, since last summer I have gained around 25-30kg (soo embarrassing but it’s a mix of health issues, mental issues and eating disorders). In winter I would wear a lot of hoodies to cover up my extra chub, but I would never feel uncomfortable because everyone else is doing the same.

This year the heat has been unbearable. We’ve had heatwaves since May and I don’t think they’re gonna stop for a while. I struggle soo much what to wear. I don’t know how to dress anymore, and with everything I do have I feel so uncomfortable with what I’m wearing because of how huge I look. Even with abayas and dresses, I feel like I look like a blob.
If I wear jeans or trousers I never know how to style the upper body like I just hate how I look.

Also because of my weight gain none of the clothes I have fit me anymore. So I need to get some more clothes which is very annoying because I’m so broke but I need clothes that are halal to wear in this heat. I don’t want something that will make me need layers eg.

I actually don’t know what to do or how to dress.
I also feel like I just keep rewearing the same clothes because I don’t have anything else, and i know everyone just keeps staring at me thinking what a weirdo.
So yeh :-)

Hi guys!

This might be bit of a silly question, but as a Brit (who is coming to Harvard soon!!) I get quite confused.

Are clubs and societies at Harvard (and the US in general) distinguished between undergrad and postgrads?

In England, a society is for all ages (unless specified for grad). We don’t really have separate events for undergrads or postgrads - is it like this at Harvard?

For context, I did my Master's in London, but I commuted two hours each way every day because I couldn't afford to live there. I was ill for my entire undergrad experience and so stayed close to home but i was SO excited to do my masters in London , but I just feel like i’ve missed out on so much. I know there’s so much of life to still come and it will only get better but I grieve this experience I never had and that i’ve always wanted.
People always tell me, "But at least you got to experience London."
The thing is... I didn't.
My university was about ten minutes from the train station. Every day I'd get off the train, go to lectures or the lab, and then head straight back because if I stayed too long I'd miss the last train home (my last train available home was 8:30pm). My life became a cycle of station ,university ,station.
I never got to wander around the city after class. I never got to stay in the library until midnight because everyone was trying to finish an assignment together. I couldn't go to last-minute socials, society events, or drinks after lectures without calculating whether it was worth being stranded. I missed networking events because of trains. I missed opportunities to build friendships because everyone else's day continued after mine had to end. And ultimately I ended up being the ‘outlier’ and everyone else was more closer to each other than me.
It wasn't just that I commuted. It felt like I was living on the outside of university life, looking in.
What hurts most is that I'll never get that year back.
Even if I move to London one day(and I hope I do)it won't be the same. I'll never get to experience being a student there. I'll never get to pull an all-nighter in the library with friends, complain about deadlines over cheap takeaway, decide at 9pm to grab coffee or go have some fun because no one has to catch a train, or simply exist in that environment where your whole life revolves around learning and the people you're learning with.
That chapter has closed.
I know there are bigger problems in the world, and I'm grateful that I even got to study for a Master's. I achieved things I'm proud of. But I can't help mourning the version of that year I imagined. The one where I wasn't constantly watching the clock. The one where I felt like I belonged to the university instead of just passing through it.
I think what I'm grieving isn't London.
It's the person I might have become if I'd actually been able to live the student life.
Has anyone else ever felt like this? Like you've lost something that technically never existed, but still feels like a real loss?

Hi guys , I’m a masters student conducting a project that requires flow cytometry. I had been using a dongle to use flowjo at home but my lab is now moving to an online software and will have to destroy the dongles which means I no longer have access to flowjo, especially because I don’t have an email address or license associated with this lab.

Does flow jo offer any free trials? Everytime I make an account I’m unable to sign up For the free trial, and can’t really afford it
I have around two months left of my project , don’t really know what I’m doing wrong

Let’s say i want to earn extra knowledge in a subject that is unrelated to my level of knowledge but will help my PhD, would this be possible?