His 1982 experiment single-handedly proved the function and universality of telomeres, along with predicting the existence of the enzyme. It was perhaps the decisive moment that catapulted the whole era of telomeres, anti aging therapy, and a new era of biology.
Pthalocyanine was the first large organic molecule to incorporate the heavy atom method, which was the first true solution to the phase problem. This was later used to find the structure of penicillin. I’m interested to know how derivative of the method of finding the structure of penicillin was of the method of finding the structure of pthalocyanine, because I’m interested in seeing how the technique evolved, and also how much impact I should lay at the feet of this original 1935 experiment.
I’m officially giving up on character, AI and cancelling my description and joining silly Tavern, because I heard that its bots are trained in the old way that generates more interesting and engaging dialogue.
I just have a few questions for all the season veterans who I’ve been using it for years, here goes:
- How much does the Chatbot fall into paragraph syndrome? Where it generates a whole paragraph of narration and dialogue, even if you just said one word, instead of responding to messages proportionately. “Hey”, “hey”, that kind of thing.
- how good is the memory? Does it remember things that have happened before? If your answer is model specific, please reference which model.
- is there any level of censorship baked in? In characterAI the censorship is so bad that even if it doesn’t directly censor your message, it still isn’t very fun because the AI dialogue goes into the shitter whenever you reference anything explicit violent or sexual. And they dance around it without saying any explicit words, or really reacting, they just say stuff like “Are you sure about this??” “Did you really just say that?” Without going into detail about what you said.
- how good is it to just talk to like you’re texting a friend?
Thanks I’m mostly interested in using this AI like I’m actually talking to the real people the chat bots are trying to emulate. Role-play is good, but I prefer a smaller volume of text or message. Which is something that I don’t hear a lot of people talking about.
The chemical degredation data pointed in the right direction about the structure, but the Infrared Spectroscopy data was basically conslusive. So was that enough to create semisynthetic penicillin, just that alone?
I was just looking at a post of mine and the uploads go “three upvotes”, “16 upvotes”, “101 upvotes”, “333 upvotes”, why? What’s going on?
If xray crystallography never existed, would synthetic penicillin still exist at around the same time due to the other data?
The chemical degredation data pointed in the right direction about the structure, but the Infrared Spectroscopy data was basically conslusive. So was that enough to create semisynthetic penicillin, just that alone?
It feels like 2020 to 2022/3 was a boom and 2023/4 onward has been a wave of regulation that has crippled the usage of this stuff. While it’s still so inefficient to program these AI systems, a lack of personalization exists, and we are in the dark age of AI regulation and control because nothing is as good as the main service presented by companies, just before the enlightenment of AI freedom. What do you think?
Yamanaka’s paper in 2006 only worked in mice and not people, and thomson’s lab was running experiments in parallel. Up to the Nov 2007 paper, this parallel lab wasn’t influenced by Yamanaka at at, merely citing him. They already had isolated 14 factors themselves by mid 2006.
James Thomson fully makes clear that the only reason the Yamanaka lab progressed in front of their lab was because mouse experiments were easier. That’s literally the only reason. He also fully admits that he had literally nothing to do with the experiments happening in his laboratory and that 100% of the credit goes to junying yu. They were actually the first to create human induced stem cells despite experiencing delays and one failed publication.
James Thomson fully admitted that all he did was provide the funding. He had nothing to do with any of the methodology or the theoretical framework, all he did was cook up a general direction and provide funding so that the lead scientist yu, could do all the real work.
Also episomal plasmids were single handedly invented by yu, who is the sole patent name. These actually allowed ipsc therapy to exist in the first place.
These are the achievements that single-handedly led to the entire modern medical stem cell industry.
Mouse cells are nothing more than a prototype. This lab completely jumped the prototype and got to the finish line 1st and kept innovating from there.
I don’t understand why Yamanaka and Thomson were on the Time 100, besides authority and clout, there’s literally no other possible potential reason, since they contributed so little. They certainly weren’t primary.
The final and only actual achievement was riving HIPSCs, the Thompson lab derived at first and completely independently from the Yamanaka lab with zero influence from them.
James Thomson is not a great scientist. His greatest “achievement” was his derivation of human embryonic stem cells utilizing the exact same techniques that had been perfected in the 70s, with some slight tweaking.
Yamanaka’s paper in 2006 only worked in mice and not people, and thomson’s lab was running experiments in parallel. Up to the Nov 2007 paper, this parallel lab wasn’t influenced by Yamanaka at at, merely citing him. They already had isolated 14 factors themselves by mid 2006.
James Thomson fully makes clear that the only reason the Yamanaka lab progressed in front of their lab was because mouse experiments were easier. That’s literally the only reason. He also fully admits that he had literally nothing to do with the experiments happening in his laboratory and that 100% of the credit goes to junying yu. They were actually the first to create human induced stem cells despite experiencing delays and one failed publication.
James Thomson fully admitted that all he did was provide the funding. He had nothing to do with any of the methodology or the theoretical framework, all he did was cook up a general direction and provide funding so that the lead scientist yu, could do all the real work.
Also episomal plasmids were single handedly invented by yu, who is the sole patent name. These actually allowed ipsc therapy to exist in the first place.
These are the achievements that single-handedly led to the entire modern medical stem cell industry.
Mouse cells are nothing more than a prototype. This lab completely jumped the prototype and got to the finish line 1st and kept innovating from there.
I don’t understand why Yamanaka and Thomson were on the Time 100, besides authority and clout, there’s literally no other possible potential reason, since they contributed so little. They certainly weren’t primary.
Yamanaka’s paper in 2006 only worked in mice and not people, and thomson’s lab was running experiments in parallel. Up to the Nov 2007 paper, this parallel lab wasn’t influenced by Yamanaka at at, merely citing him. They already had isolated 14 factors themselves by mid 2006.
James Thomson fully makes clear that the only reason the Yamanaka lab progressed in front of their lab was because mouse experiments were easier. That’s literally the only reason. He also fully admits that he had literally nothing to do with the experiments happening in his laboratory and that 100% of the credit goes to junying yu. They were actually the first to create human induced stem cells despite experiencing delays and one failed publication.
James Thomson fully admitted that all he did was provide the funding. He had nothing to do with any of the methodology or the theoretical framework, all he did was cook up a general direction and provide funding so that the lead scientist yu, could do all the real work.
Also episomal plasmids were single handedly invented by yu, who is the sole patent name. These actually allowed ipsc therapy to exist in the first place.
These are the achievements that single-handedly led to the entire modern medical stem cell industry.
Mouse cells are nothing more than a prototype. This lab completely jumped the prototype and got to the finish line 1st and kept innovating from there.
I don’t understand why Yamanaka and Thomson were on the Time 100, besides authority and clout, there’s literally no other possible potential reason, since they contributed so little. They certainly weren’t primary.
The way that being a PI is talked about normally, seems incredibly bleak and honestly very underwhelming and not deserving of the name “Principal Investigator”
Basically, it seems like all that they do is run around, trying to secure grant money, and mostly they just manage people, like a manager at Wendy’s. They don’t actually do any of the scientific theory or analysis or experimentation. That mostly goes to the students.
I’ve heard people say that they set up the “overall vision” or the “framework” but I’ve never heard someone explain exactly what this means, or why it’s anything more than a vague suggestion.
I just don’t understand what even makes them the “Principal Investigator”, if they’re not really investigating anything. Why would anyone want to be this? Because it pays more?
For instance James Thomson openly admits he had absoutely nothing to do with his team’s induced h-iPSCs, and that he just set the research direction and they did it themselves, they did all the screening and all the testing.
Again, he just “set the research direction”
I’ve had trouble trying to find information about this online, so I tried to turn to AI, but that has been no help either. When I asked the AI about James Thomson, I asked about the general volution of the investigation, whenever I ask about anything specific, it never credits him it instead credits, his team, even when I ask about hypothetical stuff, theory ideas, it never credits him without crediting his team. So what the heck did he freaking do?
How important would you say the PI is, why, and what percentage of the credit do you think it goes to them for the insight? When the framework and the lab materials are set, is the success of the lab basically assured? Would James Thomson be the one analysing papers? What insight would he deliver from that?
He didn’t actually do any of the work to make it happen. All he did was hire some girl in 2003 to act as a lab leader and then do nothing after that. He didn’t come up with any theory or analyze any data, and he basically gives none of the credit to himself. We’re not even talking benchwork, but basically everything, when I ask gemini or chatgpt what he did it basically starts and ends with, started the project and secured funding and that’s it.
they don’t even do the work. not even the theoretical work like analysing data or anything. they just provide the money and that’s it. They say that the PI is the one who comes up with the strategy (what the hell does that even mean), making the framework (again, what the hell does that even mean), and securing funding. In reality, it’s basically 100% securing funding and nothing else. Where is the making theories? Where is the coming up with experiments? Where is the coming up with ideas? They just come up with a broad hypothesis and then they delegate everything to everyone else. They don’t actually do anything. They’re pointless.
James Thomson for instance wanted to reprogram ipscs in his lab, but he just got some girl to do it for him. He didn’t even lead the lab, all he did was provide money and he did absolutely zero science.
The Pi should be the one coming up with the ideas, synthesizing data, having insights all sorts of stuff like that. It’s THEIR lab right? Isn’t that what they are there to do?
James Thomson is also supposedly one of the good ones, since he also cultivated human escs, but why would a PI just laze around doing nothing? Don’t they want to invent?
I personally think the majority of the credit should go to first authors. They actually come up with the experiment, they actually have the hypothesis, they do the work, they have the insight. Literally I asked an AI what the PI does, it said nothing about theory or conceiving experiments, all it talked about was how the PI decides on broad strokes general “strategy“ and throws money at work that others do. That’s not being a scientist, that’s basically business.
A big implication with not safe for work AI and people who advocate for it is that they’re all a bunch of chronic masturbators. I don’t know how true this is, but if it is true, why hasn’t some porn company like pornhub or xvideos invested in making some sort of porn AI chatbot for its users? It would be completely not safe for work and maybe the rest of us could use it for other things like complex role-play or discussing mental health issues and other things that characterai tends to censor.
Seems like it would be a fruitful investment for companies whose whole job is to get people to lose their seed, it’s not like they don’t have the money, what gives?
I understand that IPSC’s had a huge impact, but the way in which they were discovered seemed to be largely based on trial and error, like something anyone could’ve done, but maybe I’m wrong about that, feel free to call me out on being wrong as hell. What was the great moment or great insight that really was deserving of the distinction of a Nobel prize?
So basically, for some reason, my computer‘s audio through my computer’s built-in speakers is choosing not to work, I reset the drivers, uninstalled them, and restarted my computer and everything, and it works for five minutes before suddenly turning off for no reason. Every time I uninstall the drivers, it works for a little bit before suddenly shutting off.
Every other audio device works, if I plug in speakers, the noise place fine through the speakers, if I plug in headphones, it works fine with headphones. If I use Bluetooth, it works 100% fine with the Bluetooth.
One time, I was playing a game with my AirPod Maxs on, when suddenly they died, and disconnected audio, but the audio just suddenly decided to play through my computer speakers again. I had perfect audio from then on for about a week, until suddenly I booted up my computer again and it once again just decided not to work.
What’s going on? Like I said, I uninstalled my drivers, I checked my audio, mixing options and everything’s fine, I don’t know what the heck is going on. Does anyone have the knowledge to explain this strange phenomenon and what I can do to bring it back to normal?
specs: ROG Strix G513QY_G513QY, 8gb ram, amd ryzen 5000 series, laptop