u/AbsoluteBatman95

• Lev from The Last of Us Part II: Lev is a trans boy who cannot medically transition due to being in a zombie apocalypse with a fascist government, yet the main cast in the story supports and affirms him as male.
• Denise Bryson from Twin Peaks: A trans woman DEA and FBI agent. She does not pass in the series, which is an integral part of her character; her boss simply tells people who don't like it to "fix their hearts or die."
• Cordelia from Vinland Saga: As a trans woman living in Northern Europe during the Viking age, it is impossible for Cordelia, who has a hulking, muscular figure, to transition to female. But despite not looking the part physically, everyone is cool with it and refers to her as female.
• Ladiva from Granblue Fantasy: A pro wrestler who makes no attempts to pass (save for hair) and is still beloved by the cast as a maternal figure, serving as a stark contrast to her species' women who are petite.

Edit: typos and incorrect lore fixes

Disney Cruella----The live action tried to paint her as a victim. The woman tried to skin 101 puppies for fur fashion.

Maleficent----Similar, the live action tries to portray her as the victim of assault.

WandaVision---She enslaves an entire town and makes them play out her "playing house" fantasies in sitcom style. There's a scene where a lady begs her to let her see her daughter again.

Wicked Witch of the West---One of the most famous antagonists, shown as the victim in the "Wicked" version.

In Real Live----Lindsay Clancy admits of to murdering her three children with an exercise band while her husband was away. Supporters have raised over one million dollars in her support.